Ribozyme against mutant K-ras mRNA suppresses tumor growth of pancreatic cancer

  • Authors:
    • Hiroshi Kijima
    • Hitoshi Yamazaki
    • Masato Nakamura
    • Kevin J. Scanlon
    • R. Y. Osamura
    • Yoshito Ueyama
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijo.24.3.559
  • Pages: 559-564
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Abstract

Point mutations in the K-ras gene are observed at a high incidence in human pancreatic carcinomas. These alterations can be used as potential targets for specific ribozyme-mediated reversal of the malignant phenotype. We designed an anti-K-ras ribozyme against codon 12 of the mutant K-ras gene transcripts (GGT↷GTT), and generated a recombinant adenovirus to express the ribozyme (rAd/anti-K-ras Rz). We inoculated Capan-1 human pancreatic carcinoma cells in athymic mice, and made Capan-1 tumor xenografts. When the Capan-1 tumors in athymic mice became approximately 100 mm3, rAd/anti-K-ras Rz was directly injected into the tumor xenografts. Fifteen (68%) of 22 tumors injected with rAd/anti-K-ras Rz showed tumor growth suppression or tumor regression; 6 of 15 tumors were completely regressive, and 1 tumor was recurrent after the tumor regression. By using the recombinant adenovirus in a mice model system, it was possible to accomplish efficient reversion of the malignant phenotype in human pancreatic tumors with K-ras gene mutation.

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March 2004
Volume 24 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kijima, H., Yamazaki, H., Nakamura, M., Scanlon, K.J., Osamura, R.Y., & Ueyama, Y. (2004). Ribozyme against mutant K-ras mRNA suppresses tumor growth of pancreatic cancer. International Journal of Oncology, 24, 559-564. https://doi.org/10.3892/ijo.24.3.559
MLA
Kijima, H., Yamazaki, H., Nakamura, M., Scanlon, K. J., Osamura, R. Y., Ueyama, Y."Ribozyme against mutant K-ras mRNA suppresses tumor growth of pancreatic cancer". International Journal of Oncology 24.3 (2004): 559-564.
Chicago
Kijima, H., Yamazaki, H., Nakamura, M., Scanlon, K. J., Osamura, R. Y., Ueyama, Y."Ribozyme against mutant K-ras mRNA suppresses tumor growth of pancreatic cancer". International Journal of Oncology 24, no. 3 (2004): 559-564. https://doi.org/10.3892/ijo.24.3.559