Effect of salivary gland adenocarcinoma cell-derived α-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor

  • Authors:
    • Takashi Matsuura
    • Takashi Uematsu
    • Minoru Yamaoka
    • Kiyofumi Furusawa
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijo.24.3.521
  • Pages: 521-528
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Abstract

The aim of this study was to clarify the effects of α-N-acetylgalactosaminidase (α-NaGalase) produced by human salivary gland adenocarcinoma (SGA) cells on the bioactivity of macrophage-activating factor (GcMAF). High exo-α-NaGalase activity was detected in the SGA cell line HSG. HSG α-NaGalase had both exo- and endo-enzyme activities, cleaving the Gal-GalNAc and GalNAc residues linked to Thr/Ser but not releasing the [NeuAc2-6]GalNac residue. Furthermore, GcMAF enzymatically prepared from the Gc protein enhanced the superoxide-generation capacity and phagocytic activity of monocytes/macrophages. However, GcMAF treated with purified α-NaGalase did not exhibit these effects. Thus, HSG possesses the capacity to produce larger quantities of α-NaGalase, which inactivates GcMAF produced from Gc protein, resulting in reduced phagocytic activity and superoxide-generation capacity of monocytes/macrophages. The present data strongly suggest that HSG α-NaGalase acts as an immunodeficiency factor in cancer patients.

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March 2004
Volume 24 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Matsuura, T., Uematsu, T., Yamaoka, M., & Furusawa, K. (2004). Effect of salivary gland adenocarcinoma cell-derived α-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor. International Journal of Oncology, 24, 521-528. https://doi.org/10.3892/ijo.24.3.521
MLA
Matsuura, T., Uematsu, T., Yamaoka, M., Furusawa, K."Effect of salivary gland adenocarcinoma cell-derived α-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor". International Journal of Oncology 24.3 (2004): 521-528.
Chicago
Matsuura, T., Uematsu, T., Yamaoka, M., Furusawa, K."Effect of salivary gland adenocarcinoma cell-derived α-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor". International Journal of Oncology 24, no. 3 (2004): 521-528. https://doi.org/10.3892/ijo.24.3.521