Involvement of the mitogen-activated protein kinase kinase 2 in the induction of cell dissociation in pancreatic cancer

  • Authors:
    • Xiaodong Tan
    • Hiroshi Egami
    • Hidenobu Kamohara
    • Shinji Ishikawa
    • Takashi Kurizaki
    • Naoya Yoshida
    • Yasuhiko Tamori
    • Eiji Takai
    • Masahiko Hirota
    • Michio Ogawa
  • View Affiliations

  • Published online on: January 1, 2004     https://doi.org/10.3892/ijo.24.1.65
  • Pages: 65-73
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Abstract

In our previous investigation, mitogen-activated protein kinase kinase 2 (MEK2) was detected as a factor which was correlated to the potential of invasion-metastasis. In this study, the immunocytochemical, immunohistochemical and mRNA expressions of MEK2 were examined in pancreatic cancer cell lines and tissue samples, respectively. Constitutive expressions of MEK2 and phosphorylated MEK (p-MEK) were observed in PC-1.0 and ASPC-1 cells, which exhibited a growth pattern of single cells, whereas the relevant expressions were quite faint in PC-1 cells and CAPAN-2 cells, which exhibited a growth pattern of island-like clonies. Simultaneous inductions of MEK2 expressions and cell dissociation were observed after the treatment with a conditioned medium (CM) of PC-1.0 cells. The expression of MEK2 and p-MEK were reduced and the cell aggregation was found in PC-1.0 and ASPC-1 cells after U0126 (a MEK inhibitor) treatment. In vivo, both the MEK2 and p-MEK overexpressed in human pancreatic cancer tissues and p-MEK was found to be more strongly expressed in the invasive front than that in the center of tumor (P<0.05). MEK2 is closely related to pancreatic cancer cell dissociation. MEK2 activation is probably involved in the first step of the cascade in the invasion-metastasis of pancreatic cancer.

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January 2004
Volume 24 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Tan, X., Egami, H., Kamohara, H., Ishikawa, S., Kurizaki, T., Yoshida, N. ... Ogawa, M. (2004). Involvement of the mitogen-activated protein kinase kinase 2 in the induction of cell dissociation in pancreatic cancer. International Journal of Oncology, 24, 65-73. https://doi.org/10.3892/ijo.24.1.65
MLA
Tan, X., Egami, H., Kamohara, H., Ishikawa, S., Kurizaki, T., Yoshida, N., Tamori, Y., Takai, E., Hirota, M., Ogawa, M."Involvement of the mitogen-activated protein kinase kinase 2 in the induction of cell dissociation in pancreatic cancer". International Journal of Oncology 24.1 (2004): 65-73.
Chicago
Tan, X., Egami, H., Kamohara, H., Ishikawa, S., Kurizaki, T., Yoshida, N., Tamori, Y., Takai, E., Hirota, M., Ogawa, M."Involvement of the mitogen-activated protein kinase kinase 2 in the induction of cell dissociation in pancreatic cancer". International Journal of Oncology 24, no. 1 (2004): 65-73. https://doi.org/10.3892/ijo.24.1.65