Bioassay using epidermal keratinocytes to determine phosphorylation status of the epidermal growth factor receptor in distant neoplasms

  • Authors:
    • Corazon D. Bucana
    • Isaiah J. Fidler
  • View Affiliations

  • Published online on: January 1, 2004     https://doi.org/10.3892/ijo.24.1.19
  • Pages: 19-24
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We developed a bioassay to evaluate the phosphorylation status of a fibrosarcoma following systemic administration of the protein tyrosine kinase inhibitor PKI 166. Samples of subcutaneous fibrosarcomas and distant skin were fixed in formalin, sectioned, and stained with several fluorescent antibodies against the epidermal growth factor receptor (EGF-R) and phosphorylated EGF-R. In mice given different doses of PKI 166, the dose-dependent inhibition of phosphorylation of EGF-R in epidermal keratinocytes paralleled that in fibrosarcomas growing subcutaneously, suggesting that skin biopsies can be used as surrogate tissues for distant neoplasms to determine the phosphorylation status of protein tyrosine kinase receptors.

Related Articles

Journal Cover

January 2004
Volume 24 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Bucana, C.D., & Bucana, C.D. (2004). Bioassay using epidermal keratinocytes to determine phosphorylation status of the epidermal growth factor receptor in distant neoplasms. International Journal of Oncology, 24, 19-24. https://doi.org/10.3892/ijo.24.1.19
MLA
Bucana, C. D., Fidler, I. J."Bioassay using epidermal keratinocytes to determine phosphorylation status of the epidermal growth factor receptor in distant neoplasms". International Journal of Oncology 24.1 (2004): 19-24.
Chicago
Bucana, C. D., Fidler, I. J."Bioassay using epidermal keratinocytes to determine phosphorylation status of the epidermal growth factor receptor in distant neoplasms". International Journal of Oncology 24, no. 1 (2004): 19-24. https://doi.org/10.3892/ijo.24.1.19