Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers

  • Authors:
    • Tetsuo Ohta
    • Koichi Shimizu
    • Shuangqin Yi
    • Hiroyuki Takamura
    • Kohji Amaya
    • Hirohisa Kitagawa
    • Masato Kayahara
    • Itasu Ninomiya
    • Sachio Fushida
    • Takashi Fujimura
    • Gen-Ichi Nishimura
    • Koichi Miwa
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/ijo.23.1.61
  • Pages: 61-66
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Abstract

Protease-activated receptor (PAR)-2 is a G protein-coupled receptor that is activated by trypsin. The purpose of this study was to examine PAR-2 expression and the role of trypsin in cell proliferation in human pancreatic cancer cells. All four pancreatic cancer cell lines studied, from well to undifferentiated types, AsPC-1, BxPC-3, Panc-1, and MIAPaCa-2, had significant levels of PAR-2 mRNA detected by reverse transcription-polymerase chain reaction, and showed a band of about 55 kDa corresponding to the known molecular weight of PAR-2: AsPC-1, BxPC-3 and Panc-1 showed a strong band, and MIAPaCa-2 showed a weak one. Immunocytochemically, AsPC-1, BxPC-3, and Panc-1 showed intense immunostaining for PAR-2, predominantly in the plasma membrane, while in MIAPaCa-2, immunostaining was weak. Proliferative activity of AsPC-1 cells was increased by concentrations of trypsin as low as 10 nM, and activity peaked at a concentration of 100 nM, representing almost 60% of that induced by 10% fetal bovine serum. In contrast, trypsin had no significant effect on proliferation of MIAPaCa-2 cells. These findings suggest that trypsin plays a role in the growth of PAR-2-positive pancreatic cancer cells and serves as a potent mitogen in vitro, functioning as a growth factor.

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July 2003
Volume 23 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ohta, T., Shimizu, K., Yi, S., Takamura, H., Amaya, K., Kitagawa, H. ... Miwa, K. (2003). Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers. International Journal of Oncology, 23, 61-66. https://doi.org/10.3892/ijo.23.1.61
MLA
Ohta, T., Shimizu, K., Yi, S., Takamura, H., Amaya, K., Kitagawa, H., Kayahara, M., Ninomiya, I., Fushida, S., Fujimura, T., Nishimura, G., Miwa, K."Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers". International Journal of Oncology 23.1 (2003): 61-66.
Chicago
Ohta, T., Shimizu, K., Yi, S., Takamura, H., Amaya, K., Kitagawa, H., Kayahara, M., Ninomiya, I., Fushida, S., Fujimura, T., Nishimura, G., Miwa, K."Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers". International Journal of Oncology 23, no. 1 (2003): 61-66. https://doi.org/10.3892/ijo.23.1.61