WD-40 repeat containing rat lethal giant larvae recessive oncogene, but not m-tomosyn, restores the salt sensitivity in Saccharomyces cerevisiae

  • Authors:
    • Yong-Soo Kim
    • Hyung-Min Chung
    • Kwang-Hyun Baek
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/ijo.23.1.229
  • Pages: 229-233
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recently, we cloned rgl-1, a putative rat homologue of the Drosophila tumor suppressor oncogene lethal (2) giant larvae (l(2)gl), from the rat brain. Sequence analysis revealed that rgl-1 encodes a 1,036 amino acid polypeptide containing a domain characteristic of WD-40 repeat sequence. It has been suggested that m-tomosyn is expressed in the rat brain, encodes a 1,116 amino acid polypeptide, contains WD-40 repeat sequence, and is homologous to the Drosophila l(2)gl and mouse mgl-1 genes. The comparison of amino acid sequence for m-tomosyn with l(2)gl and mgl-1 proteins using the clustal method showed only 17.8% and 20% amino acid identity, respectively. However, amino acid identity of rgl-1 protein to l(2)gl and mgl-1 proteins is much higher (30.6% and 96.8%, respectively). Therefore, we investigated their biological function in the absence of Saccharomyces cerevisiae SOP1 and SOP2, the yeast homologues of the Drosophila l(2)gl. Functional analysis showed that the expression of the rgl-1 cDNA, but not m-tomosyn, restored partially salt sensitivity in yeast, indicating that rgl-1 is a homologue of lgl family members.

Related Articles

Journal Cover

July 2003
Volume 23 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kim, Y., Chung, H., & Baek, K. (2003). WD-40 repeat containing rat lethal giant larvae recessive oncogene, but not m-tomosyn, restores the salt sensitivity in Saccharomyces cerevisiae. International Journal of Oncology, 23, 229-233. https://doi.org/10.3892/ijo.23.1.229
MLA
Kim, Y., Chung, H., Baek, K."WD-40 repeat containing rat lethal giant larvae recessive oncogene, but not m-tomosyn, restores the salt sensitivity in Saccharomyces cerevisiae". International Journal of Oncology 23.1 (2003): 229-233.
Chicago
Kim, Y., Chung, H., Baek, K."WD-40 repeat containing rat lethal giant larvae recessive oncogene, but not m-tomosyn, restores the salt sensitivity in Saccharomyces cerevisiae". International Journal of Oncology 23, no. 1 (2003): 229-233. https://doi.org/10.3892/ijo.23.1.229