Multiple tumor marker analyses (PSA, hK2, PSCA, trp-p8) in primary prostate cancers using quantitative RT-PCR

  • Authors:
    • Susanne Fuessel
    • Denise Sickert
    • Axel Meye
    • Ulrich Klenk
    • Uta Schmidt
    • Marc Schmitz
    • Anne-Katrin Rost
    • Bernd Weigle
    • Andrea Kiessling
    • Manfred P. Wirth
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/ijo.23.1.221
  • Pages: 221-228
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Abstract

The identification of new diagnostic markers and potential treatment targets for prostate carcinoma (PCa) necessitates the evaluation of expression patterns in both malignant and non-malignant tissue specimens. In this study, we compared the mRNA expression of recently identified prostate-associated genes, prostate stem cell antigen (PSCA) and transient receptor potential p8 (trp-p8), to the mRNA expression of the most commonly used markers for PCa, prostate-specific antigen (PSA) and human kallikrein 2 (hK2). For these four candidates we performed highly specific quantitative real-time LightCycler RT-PCR assays with cDNA originating from matched tissue specimens of 40 patients with primary PCa. The highest transcript amounts were found for PSA in malignant as well as in non-malignant tissue specimens followed by hK2, trp-p8 and PSCA with an mRNA expression remarkably lower. The relative transcript levels of PSA, hK2 and trp-p8 were elevated in malignant in comparison to non-malignant tissues, but only for trp-p8 this increased expression was statistically significant. Focussing on organ confined tumors, we found a significant difference of the mRNA expression of PSA and trp-p8 between malignant and non-malignant tissue specimens. The marker trp-p8 is also suited to differentiate between the tumor stages when quantifying its transcript levels within tumor tissue specimens. The evaluation of the mRNA expression patterns of these markers by quantitative real-time RT-PCR could provide new tools for differential diagnosis and molecular staging. According to our data, the novel marker trp-p8 seems to represent a highly prostate-specific and PCa-associated gene qualifying it as a potential target for specific therapies.

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July 2003
Volume 23 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Fuessel, S., Sickert, D., Meye, A., Klenk, U., Schmidt, U., Schmitz, M. ... Wirth, M.P. (2003). Multiple tumor marker analyses (PSA, hK2, PSCA, trp-p8) in primary prostate cancers using quantitative RT-PCR. International Journal of Oncology, 23, 221-228. https://doi.org/10.3892/ijo.23.1.221
MLA
Fuessel, S., Sickert, D., Meye, A., Klenk, U., Schmidt, U., Schmitz, M., Rost, A., Weigle, B., Kiessling, A., Wirth, M. P."Multiple tumor marker analyses (PSA, hK2, PSCA, trp-p8) in primary prostate cancers using quantitative RT-PCR". International Journal of Oncology 23.1 (2003): 221-228.
Chicago
Fuessel, S., Sickert, D., Meye, A., Klenk, U., Schmidt, U., Schmitz, M., Rost, A., Weigle, B., Kiessling, A., Wirth, M. P."Multiple tumor marker analyses (PSA, hK2, PSCA, trp-p8) in primary prostate cancers using quantitative RT-PCR". International Journal of Oncology 23, no. 1 (2003): 221-228. https://doi.org/10.3892/ijo.23.1.221