Specific COX-2 inhibitor, NS-398, suppresses cellular proliferation and induces apoptosis in human hepatocellular carcinoma cells

  • Authors:
    • Alfred S.L. Cheng
    • Henry L.Y. Chan
    • Wai K. Leung
    • Nathalie Wong
    • Philip J. Johnson
    • Joseph J.Y. Sung
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/ijo.23.1.113
  • Pages: 113-119
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Abstract

Cyclooxygenase (COX-2) has been recently suggested to play a role in hepatocarcinogenesis. However, the exact pathway by which COX-2 affects the growth of hepatocellular carcinoma (HCC) is not clear. This study investigated the effects of a specific COX-2 inhibitor, NS-398, on the cell proliferation and apoptosis of COX-2-expressing and non-expressing HCC cell lines. In addition, the modulatory effect of NS-398 on apoptosis-regulating gene expression was examined. Semi-quantitative/quantitative reverse transcription-polymerase chain reaction and Western blot showed that Hep3B and HKCI-4 cells expressed COX-2 mRNA and protein, but HepG2 cells did not. NS-398 suppressed cell proliferation and induced apoptosis in the two COX-2-expressing cell lines in a dose-dependent manner, but not in HepG2 cells. Fas ligand mRNA and protein expression were increased by the treatment with NS-398 (10 µM) in COX-2-expressing cell lines. The expressions of Fas and Bcl-2 family genes (Bax, Bcl-2, Bcl-xL, Bcl-xS) were not affected by NS-398 treatment in all three cell lines. In conclusion, specific COX-2 inhibitor suppresses cell proliferation and induces apoptosis in HCC cell lines that express COX-2. Our finding suggests that COX-2 inhibition may offer a new approach for HCC chemoprevention.

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July 2003
Volume 23 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Cheng, A.S., Chan, H.L., Leung, W.K., Wong, N., Johnson, P.J., & Sung, J.J. (2003). Specific COX-2 inhibitor, NS-398, suppresses cellular proliferation and induces apoptosis in human hepatocellular carcinoma cells. International Journal of Oncology, 23, 113-119. https://doi.org/10.3892/ijo.23.1.113
MLA
Cheng, A. S., Chan, H. L., Leung, W. K., Wong, N., Johnson, P. J., Sung, J. J."Specific COX-2 inhibitor, NS-398, suppresses cellular proliferation and induces apoptosis in human hepatocellular carcinoma cells". International Journal of Oncology 23.1 (2003): 113-119.
Chicago
Cheng, A. S., Chan, H. L., Leung, W. K., Wong, N., Johnson, P. J., Sung, J. J."Specific COX-2 inhibitor, NS-398, suppresses cellular proliferation and induces apoptosis in human hepatocellular carcinoma cells". International Journal of Oncology 23, no. 1 (2003): 113-119. https://doi.org/10.3892/ijo.23.1.113