Restoration of p53 gene function in 12-O-tetradecanoylphorbor 13-acetate-resistant human leukemia K562/TPA cells

  • Authors:
    • Jitsuo Usuda
    • Motoko Inomata
    • Hisao Fukumoto
    • Yasuo Iwamoto
    • Toshihiro Suzuki
    • Hyo-Jeong Kuh
    • Kazuya Fukuoka
    • Harubumi Kato
    • Nagahiro Saijo
    • Kazuto Nishio
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/ijo.22.1.81
  • Pages: 81-86
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Abstract

The human leukemia K562 cell line does not express wild-type p53 protein. Due to the loss of one p53 allele and an insertion mutation in exon 5 of the other allele resulting in a frameshift mutation, K562 cells express a truncated p53 protein of 148 amino acids. A human leukemia phorbol ester-resistant subline, K562/TPA, is cross-resistant to some anticancer agents. A remarkable difference in cell cycle progression at G1/S phase was observed in the synchronised K562/TPA cells as compared with K562 cells. Southern blot and DNA sequence analysis revealed no mutation in exon 5 of the p53 gene in K562/TPA cells. p21Cip1 expression was also restored in K562/TPA cells confirming that the reversal of this p53 gene mutation restored wild-type p53 function in these cells. This is a unique report describing reversal of p53 gene mutation by drugs. This was associated with the expression of wild-type p53 mRNA and protein in K562/TPA cells. The K562/TPA cell line may provide a very useful tool for the investigation of the relationship between p53 status and chemosensitization.

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January 2003
Volume 22 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Usuda, J., Inomata, M., Fukumoto, H., Iwamoto, Y., Suzuki, T., Kuh, H. ... Nishio, K. (2003). Restoration of p53 gene function in 12-O-tetradecanoylphorbor 13-acetate-resistant human leukemia K562/TPA cells. International Journal of Oncology, 22, 81-86. https://doi.org/10.3892/ijo.22.1.81
MLA
Usuda, J., Inomata, M., Fukumoto, H., Iwamoto, Y., Suzuki, T., Kuh, H., Fukuoka, K., Kato, H., Saijo, N., Nishio, K."Restoration of p53 gene function in 12-O-tetradecanoylphorbor 13-acetate-resistant human leukemia K562/TPA cells". International Journal of Oncology 22.1 (2003): 81-86.
Chicago
Usuda, J., Inomata, M., Fukumoto, H., Iwamoto, Y., Suzuki, T., Kuh, H., Fukuoka, K., Kato, H., Saijo, N., Nishio, K."Restoration of p53 gene function in 12-O-tetradecanoylphorbor 13-acetate-resistant human leukemia K562/TPA cells". International Journal of Oncology 22, no. 1 (2003): 81-86. https://doi.org/10.3892/ijo.22.1.81