Bcl-2 in cancer and normal tissue cells as a prediction marker of response to 5-fluorouracil

  • Authors:
    • Masao Sasaki
    • Tsutomu Kumazaki
    • Keiji Tanimoto
    • Masahiko Nishiyama
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/ijo.22.1.181
  • Pages: 181-186
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Abstract

Bcl-2 in cancer cells was shown to be a potent indicator of 5-FU efficacy, but the protein in normal tissue cells appeared not to be a marker of 5-FU toxicity probably due to the functional alteration of Bcl-2 associated with cell senescence. Transfection analysis of Bcl-2-S and Bcl-2-AS into A549 lung cancer cells revealed that Bcl-2 suppressed cell death induced by 5-FU, and the gene expression level of Bcl-2 was closely correlated with the IC50 for 5-FU in 21 fresh human gastric tumor specimens. Such correlation could not be observed in a neonatal human foreskin fibroblast strain, MJ90 (HCA2), and 21 human normal tissues adjacent to tumors. Transfection analysis of Bcl-2-S and Bcl-2-AS into MJ90 cells showed that Bcl-2 correlated with the resistance to 5-FU in the transfectants at PDL60 as in A549 cells, but increased Bcl-2 in the PDL72 senescent transfectant did not cause an increase of the resistance to 5-FU. Cell aging was observed in MJ90 cells and Bcl-2 in the cells was found to decrease with the cell senescence. The senescent cells, however, were more resistant to 5-FU than the younger PDL60 cells having proliferation activity.

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January 2003
Volume 22 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Sasaki, M., Kumazaki, T., Tanimoto, K., & Nishiyama, M. (2003). Bcl-2 in cancer and normal tissue cells as a prediction marker of response to 5-fluorouracil. International Journal of Oncology, 22, 181-186. https://doi.org/10.3892/ijo.22.1.181
MLA
Sasaki, M., Kumazaki, T., Tanimoto, K., Nishiyama, M."Bcl-2 in cancer and normal tissue cells as a prediction marker of response to 5-fluorouracil". International Journal of Oncology 22.1 (2003): 181-186.
Chicago
Sasaki, M., Kumazaki, T., Tanimoto, K., Nishiyama, M."Bcl-2 in cancer and normal tissue cells as a prediction marker of response to 5-fluorouracil". International Journal of Oncology 22, no. 1 (2003): 181-186. https://doi.org/10.3892/ijo.22.1.181