Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells

  • Authors:
    • Myung-Jin Park
    • In-Chul Park
    • Hyung-Chan Lee
    • Sang-Hyeok Woo
    • Jae-Young Lee
    • Young-Joon Hong
    • Chang-Hun Rhee
    • Yun-Sil Lee
    • Seung-Hoon Lee
    • Bum-Sang Shim
    • Toshino Kuroki
    • Seok-Il Hong
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/ijo.22.1.137
  • Pages: 137-143
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Abstract

In the present study, phorbol 12-myristate 13-acetate (PMA) was found to increase secretion of matrix metalloproteinase (MMP)-9 and in vitro invasion in bovine capillary endothelial (BCE) cells, which were blocked by specific inhibitors of protein kinase C (PKC). To elucidate molecular mechanisms involved, we studied the effect of PMA on the activation of mitogen activated protein kinases (MAPKs), and found that PMA activated extracellular signal-regulated kinase (ERK)1/2 and PD98059, a specific inhibitor of MAPK kinase, significantly reduced PMA-induced MMP-9 secretion as well as in vitro invasion of BCE cells. Treatment of safingol, a specific PKC-α inhibitor, and introduction of antisense PKC-α into these cells reduced the secretion of MMP-9 and activation of ERK1/2 by PMA. Furthermore, we employed adenoviral PKC-α and found that weak PMA stimulation (5 ng/ml) enhanced ERK1/2 activation and MMP-9 secretion in these cells. Therefore, we strongly suggest that PKC-α, partly at least, have a crucial role in MMP-9 secretion and invasion of BCE cells which are mediated via ERK1/2 signaling pathway.

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January 2003
Volume 22 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Park, M., Park, I., Lee, H., Woo, S., Lee, J., Hong, Y. ... Hong, S. (2003). Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells. International Journal of Oncology, 22, 137-143. https://doi.org/10.3892/ijo.22.1.137
MLA
Park, M., Park, I., Lee, H., Woo, S., Lee, J., Hong, Y., Rhee, C., Lee, Y., Lee, S., Shim, B., Kuroki, T., Hong, S."Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells". International Journal of Oncology 22.1 (2003): 137-143.
Chicago
Park, M., Park, I., Lee, H., Woo, S., Lee, J., Hong, Y., Rhee, C., Lee, Y., Lee, S., Shim, B., Kuroki, T., Hong, S."Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells". International Journal of Oncology 22, no. 1 (2003): 137-143. https://doi.org/10.3892/ijo.22.1.137