Engagement of PI-3-kinase mediated Protein kinase C ζ activation in protecting Friend cells from ionizing radiation-induced apoptosis

  • Authors:
    • Amelia Cataldi
    • Roberta Di Pietro
    • Lucia Centurione
    • Monica Rapino
    • Eugenio Santavenere
    • Francesco Garaci
    • Lucio Cocco
    • Gabriella Giuliani-Piccari
    • Rosalba Rana
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/ijo.22.1.129
  • Pages: 129-135
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Abstract

Murine erythroleukemia cells (Friend) respond to ionizing radiation with the activation and nuclear translocation of p85α subunit of phosphatidylinositol-3-kinase (PI-3-kinase) which mediates the downstream activation and nuclear translocation of atypical Protein kinase C ζ (PKC ζ). This event occurs mainly upon high dose of ionizing radiation (15 Gy) and is concomitant to an increase in BrdU incorporation, which probably accounts for a predominant repair DNA synthesis. Following treatment with wortmannin, a relatively specific inhibitor of PI-3-kinase, both an increased number of apoptotic cells and the inhibition of protein kinase C ζ translocation were detected. Altogether the evidence suggests a potential role of the PI-3-kinase/PKC ζ pathway in protecting Friend cells from ionizing radiation-induced apoptosis offering PKC ζ for consideration as possible target of pharmacological treatments.

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January 2003
Volume 22 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Cataldi, A., Di Pietro, R., Centurione, L., Rapino, M., Santavenere, E., Garaci, F. ... Rana, R. (2003). Engagement of PI-3-kinase mediated Protein kinase C ζ activation in protecting Friend cells from ionizing radiation-induced apoptosis. International Journal of Oncology, 22, 129-135. https://doi.org/10.3892/ijo.22.1.129
MLA
Cataldi, A., Di Pietro, R., Centurione, L., Rapino, M., Santavenere, E., Garaci, F., Cocco, L., Giuliani-Piccari, G., Rana, R."Engagement of PI-3-kinase mediated Protein kinase C ζ activation in protecting Friend cells from ionizing radiation-induced apoptosis". International Journal of Oncology 22.1 (2003): 129-135.
Chicago
Cataldi, A., Di Pietro, R., Centurione, L., Rapino, M., Santavenere, E., Garaci, F., Cocco, L., Giuliani-Piccari, G., Rana, R."Engagement of PI-3-kinase mediated Protein kinase C ζ activation in protecting Friend cells from ionizing radiation-induced apoptosis". International Journal of Oncology 22, no. 1 (2003): 129-135. https://doi.org/10.3892/ijo.22.1.129