In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone

  • Authors:
    • Ahmed M. Shoieb
    • Mona Elgayyar
    • Paul S. Dudrick
    • John L. Bell
    • Patricia K. Tithof
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/ijo.22.1.107
  • Pages: 107-113
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Abstract

Thymoquinone (TQ) is likely responsible for the chemotherapeutic effects of N. sativa extract; however, the cellular mechanisms remain ill-defined. TQ-induced cytotoxicity was investigated using canine osteosarcoma (COS31), its cisplatin-resistant variant (COS31/rCDDP), human breast adenocarcinoma (MCF7), human ovarian adenocarcinoma (BG-1) and Madin-Darby canine (MDCK) cell lines. TQ-induced cytotoxicity was determined using a proliferation assay (MTT assay) and apoptosis assays. Effects of TQ on the cell cycle were determined using flow cytometry. COS31/rCDDP resistant cells were the most sensitive cell line to TQ and MDCK cells were the least sensitive. TQ (25 µM) induced apoptosis of COS31 cells 6 h after treatment and decreased the number of COS31 cells in S-phase and increased cells in G1-phase, indicating cell cycle arrest at G1. These results suggest that TQ kills cancer cells by a process that involves apoptosis and cell cycle arrest. Non-cancerous cells are relatively resistant to TQ.

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January 2003
Volume 22 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Shoieb, A.M., Elgayyar, M., Dudrick, P.S., Bell, J.L., & Tithof, P.K. (2003). In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. International Journal of Oncology, 22, 107-113. https://doi.org/10.3892/ijo.22.1.107
MLA
Shoieb, A. M., Elgayyar, M., Dudrick, P. S., Bell, J. L., Tithof, P. K."In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone". International Journal of Oncology 22.1 (2003): 107-113.
Chicago
Shoieb, A. M., Elgayyar, M., Dudrick, P. S., Bell, J. L., Tithof, P. K."In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone". International Journal of Oncology 22, no. 1 (2003): 107-113. https://doi.org/10.3892/ijo.22.1.107