Open Access

The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth

  • Authors:
    • Patrick B. Thomas
    • Inge Seim
    • Penny L. Jeffery
    • Manuel D. Gahete
    • Michelle Maugham
    • Gabrielle J. Crisp
    • Andrew Stacey
    • Esha T. Shah
    • Carina Walpole
    • Eliza J. Whiteside
    • Colleen C. Nelson
    • Adrian C. Herington
    • Raúl M. Luque
    • Rakesh N. Veedu
    • Lisa K. Chopin
  • View Affiliations

  • Published online on: October 4, 2019     https://doi.org/10.3892/ijo.2019.4891
  • Copyright: © Thomas et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent evidence suggests that numerous long non‑coding RNAs (lncRNAs) are dysregulated in cancer, and have critical roles in tumour development and progression. The present study investigated the ghrelin receptor antisense lncRNA growth hormone secretagogue receptor opposite strand (GHSROS) in breast cancer. Reverse transcription‑quantitative polymerase chain reaction revealed that GHSROS expression was significantly upregulated in breast tumour tissues compared with normal breast tissue. Induced overexpression of GHSROS in the MDA‑MB‑231 breast cancer cell line significantly increased cell migration in vitro, without affecting cell proliferation, a finding similar to our previous study on lung cancer cell lines. Microarray analysis revealed a significant repression of a small cluster of major histocompatibility class II genes and enrichment of immune response pathways; this phenomenon may allow tumour cells to better evade the immune system. Ectopic overexpression of GHSROS in the MDA‑MB‑231 cell line significantly increased orthotopic xenograft growth in mice, suggesting that in vitro culture does not fully capture the function of this lncRNA. This study demonstrated that GHSROS may serve a relevant role in breast cancer. Further studies are warranted to explore the function and therapeutic potential of this lncRNA in breast cancer progression.

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APA
Thomas, P.B., Seim, I., Jeffery, P.L., Gahete, M.D., Maugham, M., Crisp, G.J. ... Chopin, L.K. (1899). The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth. International Journal of Oncology, 0, 0-0. https://doi.org/10.3892/ijo.2019.4891
MLA
Thomas, P. B., Seim, I., Jeffery, P. L., Gahete, M. D., Maugham, M., Crisp, G. J., Stacey, A., Shah, E. T., Walpole, C., Whiteside, E. J., Nelson, C. C., Herington, A. C., Luque, R. M., Veedu, R. N., Chopin, L. K."The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth". International Journal of Oncology 0.0 (1899): 0-0.
Chicago
Thomas, P. B., Seim, I., Jeffery, P. L., Gahete, M. D., Maugham, M., Crisp, G. J., Stacey, A., Shah, E. T., Walpole, C., Whiteside, E. J., Nelson, C. C., Herington, A. C., Luque, R. M., Veedu, R. N., Chopin, L. K."The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth". International Journal of Oncology 0, no. 0 (1899): 0-0. https://doi.org/10.3892/ijo.2019.4891