Autocrine secretions enhance radioresistance in an exosome‑independent manner in NSCLC cells

  • Authors:
    • Shuang Wang
    • Piaoyang Gao
    • Na Li
    • Ping Chen
    • Jinhan  Wang
    • Ningning He
    • Kaihua Ji
    • Liqing Du
    • Qiang Liu
  • View Affiliations

  • Published online on: November 2, 2018     https://doi.org/10.3892/ijo.2018.4620
  • Pages: 229-238
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Abstract

Radiotherapy resistance in patient with non‑small cell lung cancer (NSCLC) reduces patient survival and remains a significant challenge for the treatment of NSCLC. Radiation resistance has been demonstrated to be affected by secreted factors, yet it remains unclear how autocrine secretions affect the radioresistance of NSCLC cells. In the present study, the NSCLC cell line, NCI‑H460, was irradiated with γ‑rays (4 Gy) and then cultured in medium from H460 cells or normal medium to examine the potential influence of cell secretions on the radiation resistance of H460 cells. Cell viability, accumulation of reactive oxygen species and DNA repair capacity were all markedly improved in the irradiated H460 cells that were cultured in conditioned medium (CM), compared with those cells cultured in normal medium. In addition, G2/M cell cycle arrest and upregulation of homologous recombination repair proteins were observed in the CM‑treated cells, while exosomes secreted by H460 cells had no influence on the radiation resistance of H460 cells. Taken together, these results indicate that autocrine secretions enhance the radiation resistance of γ‑irradiated H460 cells and that these secretions mainly affect the DNA repair process.
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January 2019
Volume 54 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Wang, S., Gao, P., Li, N., Chen, P., Wang, J., He, N. ... Liu, Q. (2019). Autocrine secretions enhance radioresistance in an exosome‑independent manner in NSCLC cells. International Journal of Oncology, 54, 229-238. https://doi.org/10.3892/ijo.2018.4620
MLA
Wang, S., Gao, P., Li, N., Chen, P., Wang, J., He, N., Ji, K., Du, L., Liu, Q."Autocrine secretions enhance radioresistance in an exosome‑independent manner in NSCLC cells". International Journal of Oncology 54.1 (2019): 229-238.
Chicago
Wang, S., Gao, P., Li, N., Chen, P., Wang, J., He, N., Ji, K., Du, L., Liu, Q."Autocrine secretions enhance radioresistance in an exosome‑independent manner in NSCLC cells". International Journal of Oncology 54, no. 1 (2019): 229-238. https://doi.org/10.3892/ijo.2018.4620