Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy

  • Authors:
    • Jung Seok Kim
    • Seong Jae Kang
    • Hwa Yeon Jeong
    • Min Woo Kim
    • Sang Il Park
    • Yeon Kyung Lee
    • Hong Sung Kim
    • Keun Sik Kim
    • Yong Serk Park
  • View Affiliations

  • Published online on: July 7, 2016     https://doi.org/10.3892/ijo.2016.3619
  • Pages: 1130-1138
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Abstract

Tumor-directed gene delivery is of major interest in the field of cancer gene therapy. Varied functionalizations of non-viral vectors have been suggested to enhance tumor targetability. In the present study, we prepared two different types of anti-EGF receptor (EGFR) immunonanoparticles containing pDNA, neutrally charged liposomes and cationic lipoplexes, for tumor-directed transfection of cancer therapeutic genes. Even though both anti-EGFR immunonanoparticles had a high binding affinity to the EGFR-positive cancer cells, the anti-EGFR immunolipoplex formulation exhibited approximately 100-fold higher transfection to the target cells than anti-EGFR immunoliposomes. The lipoplex formulation also showed a higher transfection to SK-OV-3 tumor xenografts in mice. Thus, IL12 and/or salmosin genes were loaded in the anti-EGFR immunolipoplexes and intravenously administered to mice carrying SK-OV-3 tumors. Co-transfection of IL12 and salmosin genes using anti-EGFR immunolipoplexes significantly reduced tumor growth and pulmonary metastasis. Furthermore, combinatorial treatment with doxorubicin synergistically inhibited tumor growth. These results suggest that anti-EGFR immunolipoplexes containing pDNA encoding therapeutic genes could be utilized as a gene-transfer modality for cancer gene therapy.
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September 2016
Volume 49 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kim, J.S., Kang, S.J., Jeong, H.Y., Kim, M.W., Park, S.I., Lee, Y.K. ... Park, Y.S. (2016). Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy. International Journal of Oncology, 49, 1130-1138. https://doi.org/10.3892/ijo.2016.3619
MLA
Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., Park, Y. S."Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy". International Journal of Oncology 49.3 (2016): 1130-1138.
Chicago
Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., Park, Y. S."Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy". International Journal of Oncology 49, no. 3 (2016): 1130-1138. https://doi.org/10.3892/ijo.2016.3619