Open Access

Phosphatidylinositol glycan anchor biosynthesis, class X containing complex promotes cancer cell proliferation through suppression of EHD2 and ZIC1, putative tumor suppressors

  • Authors:
    • Makoto Nakakido
    • Kenji Tamura
    • Suyoun Chung
    • Koji Ueda
    • Risa Fujii
    • Kazuma Kiyotani
    • Yusuke Nakamura
  • View Affiliations

  • Published online on: July 6, 2016     https://doi.org/10.3892/ijo.2016.3607
  • Pages: 868-876
  • Copyright: © Nakakido et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We identified phosphatidylinositol glycan anchor biosynthesis, class X (PIGX), which plays a critical role in the biosynthetic pathway of glycosylphosphatidylinositol (GPI)-anchor motif, to be upregulated highly and frequently in breast cancer cells. Knockdown of PIGX as well as reticulocalbin 1 (RCN1) and reticulocalbin 2 (RCN2), which we found to interact with PIGX and was indicated to regulate calcium-dependent activities, significantly suppressed the growth of breast cancer cells. We also identified PIGX to be a core protein in an RCN1/PIGX/RCN2 complex. Microarray analysis revealed that the expression of two putative tumor suppressor genes, Zic family member 1 (ZIC1) and EH-domain containing 2 (EHD2), were upregulated commonly in cells in which PIGX, RCN1, or RCN2 was knocked down, suggesting that this RCN1/PIGX/RCN2 complex could negatively regulate the expression of these two genes and thereby contribute to human breast carcinogenesis. Our results imply that PIGX may be a good candidate molecule for development of novel anticancer drugs for breast cancer.
View Figures
View References

Related Articles

Journal Cover

September 2016
Volume 49 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Nakakido, M., Tamura, K., Chung, S., Ueda, K., Fujii, R., Kiyotani, K., & Nakamura, Y. (2016). Phosphatidylinositol glycan anchor biosynthesis, class X containing complex promotes cancer cell proliferation through suppression of EHD2 and ZIC1, putative tumor suppressors. International Journal of Oncology, 49, 868-876. https://doi.org/10.3892/ijo.2016.3607
MLA
Nakakido, M., Tamura, K., Chung, S., Ueda, K., Fujii, R., Kiyotani, K., Nakamura, Y."Phosphatidylinositol glycan anchor biosynthesis, class X containing complex promotes cancer cell proliferation through suppression of EHD2 and ZIC1, putative tumor suppressors". International Journal of Oncology 49.3 (2016): 868-876.
Chicago
Nakakido, M., Tamura, K., Chung, S., Ueda, K., Fujii, R., Kiyotani, K., Nakamura, Y."Phosphatidylinositol glycan anchor biosynthesis, class X containing complex promotes cancer cell proliferation through suppression of EHD2 and ZIC1, putative tumor suppressors". International Journal of Oncology 49, no. 3 (2016): 868-876. https://doi.org/10.3892/ijo.2016.3607