The tumor-suppressive microRNA-23b/27b cluster regulates the MET oncogene in oral squamous cell carcinoma

  • Authors:
    • Ichiro Fukumoto
    • Keiichi Koshizuka
    • Toyoyuki Hanazawa
    • Naoko Kikkawa
    • Ryosuke Matsushita
    • Akira Kurozumi
    • Mayuko Kato
    • Atsushi Okato
    • Yoshitaka Okamoto
    • Naohiko Seki
  • View Affiliations

  • Published online on: July 4, 2016     https://doi.org/10.3892/ijo.2016.3602
  • Pages: 1119-1129
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Our recent studies of microRNA (miRNA) expression signatures in human cancers revealed that two clustered miRNAs, microRNA-23b (miR-23b) and microRNA-27b (miR‑27b), were significantly reduced in cancer tissues. Few reports have provided functional analyses of these clustered miRNAs in oral squamous cell carcinoma (OSCC). The aim of this study was to investigate the functional significance of miR-23b and miR-27b in OSCC and to identify novel miR-23b/27b-mediated cancer pathways and target genes involved in OSCC oncogenesis and metastasis. Expression levels of miR-23b and miR-27b were significantly reduced in OSCC specimens. Restoration of miR-23b or miR-27b in cancer cells revealed that both miRNAs significantly inhibited cancer cell migration and invasion. Our in silico analyses and luciferase reporter assays showed that the receptor tyrosine kinase MET, was directly regulated by these miRNAs. Moreover, downregulating the MET gene by use of siRNA significantly inhibited cell migration and invasion by OSCC cells. The identification of novel molecular pathways regulated by miR-23b and miR-27b may lead to a better understanding of the oncogenesis and metastasis of this disease.
View Figures
View References

Related Articles

Journal Cover

September 2016
Volume 49 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Fukumoto, I., Koshizuka, K., Hanazawa, T., Kikkawa, N., Matsushita, R., Kurozumi, A. ... Seki, N. (2016). The tumor-suppressive microRNA-23b/27b cluster regulates the MET oncogene in oral squamous cell carcinoma. International Journal of Oncology, 49, 1119-1129. https://doi.org/10.3892/ijo.2016.3602
MLA
Fukumoto, I., Koshizuka, K., Hanazawa, T., Kikkawa, N., Matsushita, R., Kurozumi, A., Kato, M., Okato, A., Okamoto, Y., Seki, N."The tumor-suppressive microRNA-23b/27b cluster regulates the MET oncogene in oral squamous cell carcinoma". International Journal of Oncology 49.3 (2016): 1119-1129.
Chicago
Fukumoto, I., Koshizuka, K., Hanazawa, T., Kikkawa, N., Matsushita, R., Kurozumi, A., Kato, M., Okato, A., Okamoto, Y., Seki, N."The tumor-suppressive microRNA-23b/27b cluster regulates the MET oncogene in oral squamous cell carcinoma". International Journal of Oncology 49, no. 3 (2016): 1119-1129. https://doi.org/10.3892/ijo.2016.3602