Licochalcone B induces apoptosis of human oral squamous cell carcinoma through the extrinsic- and intrinsic-signaling pathways

  • Authors:
    • Hana Oh
    • Goo Yoon
    • Jae-Cheon Shin
    • Seon-Min Park
    • Seung-Sik Cho
    • Jin Hyoung Cho
    • Mee-Hyun Lee
    • Kangdong Liu
    • Young Sik Cho
    • Jung-Il Chae
    • Jung-Hyun Shim
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  • Published online on: February 1, 2016     https://doi.org/10.3892/ijo.2016.3365
  • Pages: 1749-1757
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Abstract

Licochalcone B (Lico B), which belongs to the retrochalcone family, is isolated from the roots of Chinese licorice. Lico B has been reported to have several other useful pharmacological properties, such as anti-inflammatory, antibacterial, antioxidant, antiulcer, anticancer, and anti-metastasis activities. We elucidated the underlying mechanism by which Lico B can induce apoptosis in oral squamous cell carcinoma (OSCC). Our results showed that exposure of OSCC cells (HN22 and HSC4) to Lico B significantly inhibited cell proliferation in a time- and concentration-dependent manner. Lico B caused cell cycle arrest at G1 phase along with downregulation of cyclin D1 and upregulation of p21 and p27 proteins. Lico B also facilitated the diffusion of phospholipid phosphatidylserine (PS) from inner to outer leaflets of the plasma membrane with chromatin condensation, DNA fragmentation, accumulated sub-G1 population in a concentration-dependent manner. Moreover, Lico B promoted the generation of reactive oxygen species (ROS), which, in turn, can induce CHOP, death receptor (DR) 4 and DR5. Lico B treatment induced downregulation of anti-apoptotic proteins (Bid and Bcl-xl and Mcl-1), and up­regulation of pro-apoptotic protein (Bax). Lico B also led to the loss of mitochondrial membrane potential (MMP), resulting in cytochrome c release. As can be expected from the above results, the apoptotic protease activating factor-1 (Apaf-1) and survivin were oppositely expressed in favor of apoptotic cell death. This notion was supported by the fact that Lico B activated multi-caspases with cleavage of poly (ADP-ribose) polymerase (PARP) protein. Therefore, it is suggested that Lico B is a promising drug for the treatment of human oral cancer via the induction of apoptotic cell death.
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April 2016
Volume 48 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Oh, H., Yoon, G., Shin, J., Park, S., Cho, S., Cho, J.H. ... Shim, J. (2016). Licochalcone B induces apoptosis of human oral squamous cell carcinoma through the extrinsic- and intrinsic-signaling pathways. International Journal of Oncology, 48, 1749-1757. https://doi.org/10.3892/ijo.2016.3365
MLA
Oh, H., Yoon, G., Shin, J., Park, S., Cho, S., Cho, J. H., Lee, M., Liu, K., Cho, Y. S., Chae, J., Shim, J."Licochalcone B induces apoptosis of human oral squamous cell carcinoma through the extrinsic- and intrinsic-signaling pathways". International Journal of Oncology 48.4 (2016): 1749-1757.
Chicago
Oh, H., Yoon, G., Shin, J., Park, S., Cho, S., Cho, J. H., Lee, M., Liu, K., Cho, Y. S., Chae, J., Shim, J."Licochalcone B induces apoptosis of human oral squamous cell carcinoma through the extrinsic- and intrinsic-signaling pathways". International Journal of Oncology 48, no. 4 (2016): 1749-1757. https://doi.org/10.3892/ijo.2016.3365