Open Access

Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer

  • Authors:
    • Huishan Zhao
    • Hefen Yu
    • Tracey A. Martin
    • Yuxiang Zhang
    • Gang Chen
    • Wen G. Jiang
  • View Affiliations

  • Published online on: January 15, 2016     https://doi.org/10.3892/ijo.2016.3340
  • Pages: 929-936
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The junctional adhesion molecule (JAMs) family belongs to the immunoglobulin subfamily involved in the formation of tight junctions (TJ) in both endothelial and epithelial cells. Aberrant expression of JAM-2 is associated with cancer progression but little work has been carried out in discovering how this affects changes in cell behaviour. The present study aimed to examine the expression of JAM-2 in human colon cancer specimens and cell lines and its role in the development of colon cancer. JAM-2 expression in human colon cancer specimens (normal, n=75; cancer, n=94) and cell lines was analysed using quantitative real-time PCR and conventional RT-PCR. Colon cancer cells were stably transfected with a mammalian expression vector to overexpress JAM-2-Flag. The effect on growth, adhesion and migration following overexpression of JAM-2 was then investigated using in vitro models. TJ function was assessed using a trans-epithelial resistance assay (TER, with an EVOM voltammeter). JAM-2 was lowly expressed in colon cancer cells such as RKO, HT115. JAM-2 overexpression in RKO cells (RKO-JAM-2) and HT115 cells (HT115-JAM-2) showed retarded adhesion (P<0.05). An in vivo tumour model showed that RKO-JAM-2 had significantly reduced growth (P<0.05), invasion (P<0.05) and migration (P<0.05) as well as in HT115-JAM-2, except on proliferation and migration. Expression of JAM-2 resulted in a significant increase in TER and decrease in permeability of polarized monolayers (P<0.05). Further analysis of JAM-2 transcript levels against clinical aspects demonstrated that the decreasing JAM-2 expression correlated to disease progression, metastasis and poor survival. Taken together, JAM-2 may function as a putative tumour suppressor in the progression and metastasis of colorectal cancer.
View Figures
View References

Related Articles

Journal Cover

March 2016
Volume 48 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zhao, H., Yu, H., Martin, T.A., Zhang, Y., Chen, G., & Jiang, W.G. (2016). Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer. International Journal of Oncology, 48, 929-936. https://doi.org/10.3892/ijo.2016.3340
MLA
Zhao, H., Yu, H., Martin, T. A., Zhang, Y., Chen, G., Jiang, W. G."Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer". International Journal of Oncology 48.3 (2016): 929-936.
Chicago
Zhao, H., Yu, H., Martin, T. A., Zhang, Y., Chen, G., Jiang, W. G."Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer". International Journal of Oncology 48, no. 3 (2016): 929-936. https://doi.org/10.3892/ijo.2016.3340