Real-time quantitative RT-PCR detection of circulating tumor cells from breast cancer patients

  • Authors:
    • Maowen Guo
    • Xiaotian Li
    • Shaohua Zhang
    • Hua Song
    • Wenhui Zhang
    • Xueyi Shang
    • Yuling Zheng
    • Hua Jiang
    • Qingyu Lv
    • Yongqiang Jiang
    • Huaijie Hao
  • View Affiliations

  • Published online on: October 29, 2014     https://doi.org/10.3892/ijo.2014.2732
  • Pages: 281-289
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Abstract

Circulating tumor cells (CTCs) were recognized as novel tumor biomarker for prognostic and predictive purposes in various cancers. Various detection technologies and devices have been developed to enumerate and characterize CTCs. Most of those approaches are based on the positive enrichment strategy and immunocytological techniques. However, the sensitivity of these approaches proved to be limited in metastatic tumors and the detection of early tumor cell dissemination was problematic. In the present study, we developed a novel CTC detection method by real-time RT-PCR technique in combination of negative enrichment strategy. The developed enrichment approach could recover more than 75% of spiked breast cancer cells from peripheral blood. The detection limit of duplex real-time RT-PCR assay using KRT19 and ERBB2 as targeted genes was consistently one breast tumor cell. Moreover, CTC detection by duplex real-time RT-PCR assay had higher detection sensitivity than that by immunostaining, especially in early breast cancer. In summary, the results of the present study indicated the potential clinical utilities of CTCs identification on breast cancer by duplex real-time RT-PCR in combination with negative enrichment.
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January 2015
Volume 46 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Guo, M., Li, X., Zhang, S., Song, H., Zhang, W., Shang, X. ... Hao, H. (2015). Real-time quantitative RT-PCR detection of circulating tumor cells from breast cancer patients. International Journal of Oncology, 46, 281-289. https://doi.org/10.3892/ijo.2014.2732
MLA
Guo, M., Li, X., Zhang, S., Song, H., Zhang, W., Shang, X., Zheng, Y., Jiang, H., Lv, Q., Jiang, Y., Hao, H."Real-time quantitative RT-PCR detection of circulating tumor cells from breast cancer patients". International Journal of Oncology 46.1 (2015): 281-289.
Chicago
Guo, M., Li, X., Zhang, S., Song, H., Zhang, W., Shang, X., Zheng, Y., Jiang, H., Lv, Q., Jiang, Y., Hao, H."Real-time quantitative RT-PCR detection of circulating tumor cells from breast cancer patients". International Journal of Oncology 46, no. 1 (2015): 281-289. https://doi.org/10.3892/ijo.2014.2732