HIF2α is associated with poor prognosis and affects the expression levels of survivin and cyclin D1 in gastric carcinoma

  • Authors:
    • Wei-Wei Tong
    • Guang-Hui Tong
    • Xie-Xiao Chen
    • Hua-Chuan Zheng
    • Yin-Zhao Wang
  • View Affiliations

  • Published online on: October 22, 2014     https://doi.org/10.3892/ijo.2014.2719
  • Pages: 233-242
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hypoxia-inducible factor-2α (HIF2α) is a major determinant factor of invasion and metastasis in various tumors. It has been reported that HIF2α is overexpressed in many tumors, including gastric cancer. However, the roles of HIF2α in the progression of gastric cancer are still not clear. In this study, we first examined the levels of HIF2α in gastric cancer by using immunohistochemistry, western blot and real-time PCR analysis. The results showed that HIF2α was highly expressed in gastric cancers compared to non-neoplastic mucosa and significantly correlated with histologic grade, TNM stages and peritoneal dissemination. MTT and colony formation assay revealed HIF2α overexpression induced high proliferation in BGC823 cells and HIF2α knockdown significantly inhibited proliferation in SGC7901 cells. Furthermore, we demonstrated that HIF2α could promote migration and invasion in gastric cancer cells. The results of western blot and RT-PCR analysis indicated that Survivin, Cyclin D1, MMP2 and MMP9 are upregulated with HIF2α overexpression. Finally, similar roles of HIF2α also in vivo were demonstrated. Taken together, the present study suggested that HIF-2α was involved in proliferation, metastasis and invasion of gastric cancer cells, with the induction of Survivin, Cyclin D1, MMP2 and MMP9 expression.
View Figures
View References

Related Articles

Journal Cover

January 2015
Volume 46 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Tong, W., Tong, G., Chen, X., Zheng, H., & Wang, Y. (2015). HIF2α is associated with poor prognosis and affects the expression levels of survivin and cyclin D1 in gastric carcinoma. International Journal of Oncology, 46, 233-242. https://doi.org/10.3892/ijo.2014.2719
MLA
Tong, W., Tong, G., Chen, X., Zheng, H., Wang, Y."HIF2α is associated with poor prognosis and affects the expression levels of survivin and cyclin D1 in gastric carcinoma". International Journal of Oncology 46.1 (2015): 233-242.
Chicago
Tong, W., Tong, G., Chen, X., Zheng, H., Wang, Y."HIF2α is associated with poor prognosis and affects the expression levels of survivin and cyclin D1 in gastric carcinoma". International Journal of Oncology 46, no. 1 (2015): 233-242. https://doi.org/10.3892/ijo.2014.2719