Upregulation of CRMP4, a new prostate cancer metastasis suppressor gene, inhibits tumor growth in a nude mouse intratibial injection model

  • Authors:
    • Wei Zhou
    • Peigen Xie
    • Mao Pang
    • Bu Yang
    • Youqiang Fang
    • Tao Shu
    • Chang Liu
    • Xuan Wang
    • Liangming Zhang
    • Shangfu Li
    • Limin Rong
  • View Affiliations

  • Published online on: October 10, 2014     https://doi.org/10.3892/ijo.2014.2705
  • Pages: 290-298
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Abstract

Prostate cancer, the most commonly diagnosed male cancer in North America, has a high incidence of bone metastasis. Our previous study showed collapsin response mediator protein 4 (CRMP4) gene inhibited prostate cancer migration and invasion. In this study, we investigated whether overexpression of CRMP4 gene in prostate cancer cells inhibit tumor bone metastasis. The stable prostate cancer cells overexpressing the CRMP4 gene were constructed using lentivirus infection. Prostate cancer bone metastasis nude mouse model was built though orthotopic prostate implantation, intracardiac injection and intratibial injection with CRMP4 overexpress and control cancer cells. Small animal PET/CT scanning results showed no difference of bone metastatic capacity in orthotopic and intracardiac injection models between CRMP4 overexpression and control group, while CRMP4 overexpression inhibited tumor growth in the intratibial injection model. Moreover, our in vitro study showed CRMP4 overexpression downregulates the Neuropilin1 (NRP1) expression and upregulate the Noggin expression. Immunohistochemical staining of the hind limbs of intratibial injection model was confirmed with cytological experiments. Taken together, our research indicated CRMP4 inhibits prostate cancer cells growth in the nude mouse bone microenvironment and this effect may relate with regulation of NRP1 and Noggin expression.
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January 2015
Volume 46 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Zhou, W., Xie, P., Pang, M., Yang, B., Fang, Y., Shu, T. ... Rong, L. (2015). Upregulation of CRMP4, a new prostate cancer metastasis suppressor gene, inhibits tumor growth in a nude mouse intratibial injection model. International Journal of Oncology, 46, 290-298. https://doi.org/10.3892/ijo.2014.2705
MLA
Zhou, W., Xie, P., Pang, M., Yang, B., Fang, Y., Shu, T., Liu, C., Wang, X., Zhang, L., Li, S., Rong, L."Upregulation of CRMP4, a new prostate cancer metastasis suppressor gene, inhibits tumor growth in a nude mouse intratibial injection model". International Journal of Oncology 46.1 (2015): 290-298.
Chicago
Zhou, W., Xie, P., Pang, M., Yang, B., Fang, Y., Shu, T., Liu, C., Wang, X., Zhang, L., Li, S., Rong, L."Upregulation of CRMP4, a new prostate cancer metastasis suppressor gene, inhibits tumor growth in a nude mouse intratibial injection model". International Journal of Oncology 46, no. 1 (2015): 290-298. https://doi.org/10.3892/ijo.2014.2705