Esculetin (6,7-dihydroxycoumarin): A potential cancer chemopreventive agent through suppression of Sp1 in oral squamous cancer cells

  • Authors:
    • Jin Hyoung Cho
    • Jae-Cheon Shin
    • Jung-Jae Cho
    • Yung Hyun Choi
    • Jung-Hyun Shim
    • Jung-Il Chae
  • View Affiliations

  • Published online on: October 7, 2014     https://doi.org/10.3892/ijo.2014.2700
  • Pages: 265-271
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Abstract

Esculetin (6,7-dihydroxycoumarin), a coumarin compound, is known to inhibit proliferation and induce apoptosis in several types of human cancer cells and is regarded as a promising chemotherapeutic agent. The purpose of the present study was to investigate the anti-proliferative effects of esculetin on two oral squamous cell carcinoma (OSCC) cell lines, HN22 and HSC4, through regulation of specificity protein 1 (Sp1). We examined the apoptotic effects of esculetin were measured by MTS assay, DAPI staining, Annexin V, PI staining, RT-PCR, western blot analysis and immunocytochemistry in HN22 and HSC4 cells. Taken together, the results of the present study indicate that esculetin had anti-proliferative effect on the growth of OSCC cells (HN22 and HSC4) in a dose- and time-dependent manner. The treatment of HN22 and HSC4 cells with esculetin led to a significant reduction in growth and induced apoptosis, followed by the regulation of Sp1 and Sp1 regulatory protein. This indicates that esculetin inhibited cell growth and induced apoptosis by suppressing Sp1 in HN22 and HSC4 cells, suggesting it to be a potent anticancer drug candidate for oral cancer.
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January 2015
Volume 46 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Cho, J.H., Shin, J., Cho, J., Choi, Y.H., Shim, J., & Chae, J. (2015). Esculetin (6,7-dihydroxycoumarin): A potential cancer chemopreventive agent through suppression of Sp1 in oral squamous cancer cells. International Journal of Oncology, 46, 265-271. https://doi.org/10.3892/ijo.2014.2700
MLA
Cho, J. H., Shin, J., Cho, J., Choi, Y. H., Shim, J., Chae, J."Esculetin (6,7-dihydroxycoumarin): A potential cancer chemopreventive agent through suppression of Sp1 in oral squamous cancer cells". International Journal of Oncology 46.1 (2015): 265-271.
Chicago
Cho, J. H., Shin, J., Cho, J., Choi, Y. H., Shim, J., Chae, J."Esculetin (6,7-dihydroxycoumarin): A potential cancer chemopreventive agent through suppression of Sp1 in oral squamous cancer cells". International Journal of Oncology 46, no. 1 (2015): 265-271. https://doi.org/10.3892/ijo.2014.2700