Open Access

microRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R

  • Authors:
    • Kang Han
    • Tingbao Zhao
    • Xiang Chen
    • Na Bian
    • Tongtao Yang
    • Qiong Ma
    • Chengkui Cai
    • Qingyu Fan
    • Yong Zhou
    • Baoan Ma
  • View Affiliations

  • Published online on: July 30, 2014     https://doi.org/10.3892/ijo.2014.2571
  • Pages: 1437-1449
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Studies have shown that miR-194 functions as a tumor suppressor and is associated with tumor growth and metastasis. We studied the effects of miR-194 in osteosarcoma and the possible mechanism by which miR-194 affected the survival, apoptosis and metastasis of osteosarcoma. Both human osteosarcoma cell lines SOSP-9607 and U2-OS were transfected with recombinant lentiviruses to regulate miR-194 expression. Overexpression of miR-194 partially inhibited the proliferation, migration, and invasion of osteosarcoma cells in vitro, as well as tumor growth and pulmonary metastasis of osteosarcoma cells in vivo. Potential miR-194 target genes were predicted using bioinformatics. Luciferase reporter assay, real-time quantitative PCR and western blotting confirmed that CDH2 (N-cadherin) and IGF1R were targets of miR-194. Using real-time quantitative PCR, we evaluated the expression of miR-194 and two miR-194 target genes, CDH2 and IGF1R in osteosarcoma samples from 107 patients and 99 formalin- or paraformalin-fixed paraffin-embedded tissues. The expressions of the target genes were also examined in osteosarcoma samples using immunohistochemistry. Overexpression of miR-194 inhibited tumor growth and metastasis of osteosarcoma probably by downregulating CDH2 and IGF1R. miR-194 may prove to be a promising therapeutic agent for osteosarcoma.
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October 2014
Volume 45 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Han, K., Zhao, T., Chen, X., Bian, N., Yang, T., Ma, Q. ... Ma, B. (2014). microRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R. International Journal of Oncology, 45, 1437-1449. https://doi.org/10.3892/ijo.2014.2571
MLA
Han, K., Zhao, T., Chen, X., Bian, N., Yang, T., Ma, Q., Cai, C., Fan, Q., Zhou, Y., Ma, B."microRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R". International Journal of Oncology 45.4 (2014): 1437-1449.
Chicago
Han, K., Zhao, T., Chen, X., Bian, N., Yang, T., Ma, Q., Cai, C., Fan, Q., Zhou, Y., Ma, B."microRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R". International Journal of Oncology 45, no. 4 (2014): 1437-1449. https://doi.org/10.3892/ijo.2014.2571