CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway

  • Authors:
    • Ga Bin Park
    • Yeong Seok Kim
    • Hyun-Kyung Lee
    • Dae-Ho Cho
    • Daejin Kim
    • Dae Young Hur
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  • Published online on: September 5, 2013     https://doi.org/10.3892/ijo.2013.2091
  • Pages: 1531-1540
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Abstract

CD80 and CD86 expression is strongly regulated in B cells and is induced by various stimuli (e.g., cytokines, ligation of MHC class II and CD40 ligand). Epstein-Barr virus (EBV) infection activates B lymphocytes and transforms them into lymphoblastoid cells. However, the role of CD80 and CD86 in EBV infection of B cells remains unclear. Here, we observed that cross-linking of CD80 and CD86 in EBV-transformed B cells induced apoptosis through caspase-dependent release of apoptosis-related molecules, cytochrome c and apoptosis-inducing factor (AIF) from mitochondria, because Z-VAD-fmk (N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone) and N-acetylcysteine (NAC) blocked apoptosis and disruption of mitochondria. Stimulation of CD80 and CD86 induced expression of Fas ligand (FasL) on EBV-transformed B cells and upregulated Fas and FasL expression in IM-9 cells. Apoptosis through Fas-FasL interactions was blocked by treatment of cells with ZB4, an antagonistic anti-Fas antibody. These results suggest that the co-stimulatory molecules CD80 and CD86 induced by EBV infection stimulate apoptosis of EBV-transformed lymphoblastoid B cells via the Fas/FasL pathway.
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November 2013
Volume 43 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Park, G.B., Kim, Y.S., Lee, H., Cho, D., Kim, D., & Hur, D.Y. (2013). CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway. International Journal of Oncology, 43, 1531-1540. https://doi.org/10.3892/ijo.2013.2091
MLA
Park, G. B., Kim, Y. S., Lee, H., Cho, D., Kim, D., Hur, D. Y."CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway". International Journal of Oncology 43.5 (2013): 1531-1540.
Chicago
Park, G. B., Kim, Y. S., Lee, H., Cho, D., Kim, D., Hur, D. Y."CD80 (B7.1) and CD86 (B7.2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway". International Journal of Oncology 43, no. 5 (2013): 1531-1540. https://doi.org/10.3892/ijo.2013.2091