Open Access

Fibroblast growth factors 7 and 10 are involved in ameloblastoma proliferation via the mitogen-activated protein kinase pathway

  • Authors:
    • Yu Nakao
    • Takeshi Mitsuyasu
    • Shintaro Kawano
    • Norifumi Nakamura
    • Shiori Kanda
    • Seiji Nakamura
  • View Affiliations

  • Published online on: August 29, 2013     https://doi.org/10.3892/ijo.2013.2081
  • Pages: 1377-1384
  • Copyright: © Nakao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Ameloblastoma is an epithelial benign tumor of the odontogenic apparatus and its growth mechanisms are not well understood. Fibroblast growth factor (FGF) 3, FGF7 and FGF10, which are expressed by the neural crest-derived ectomesenchymal cells, induce the proliferation of odontogenic epithelial cells during tooth development. Therefore, we examined the expression and function of these FGFs in ameloblastoma. We examined 32 cases of ameloblastoma as well as AM-1 cells (an ameloblastoma cell line) and studied the expression of FGF3, FGF7, FGF10 and their specific receptors, namely, FGF receptor (FGFR) 1 and FGFR2. Proliferation, mitogen-activated protein kinase (MAPK) signaling and PI3K signaling were examined in AM-1 cells after the addition of FGF7, FGF10 and these neutralizing antibodies. The expression of FGF7, FGF10, FGFR1 and FGFR2 was detected in ameloblastoma cells and AM-1 cells, while that of FGF3 was not. FGF7 and FGF10 stimulated AM-1 cell proliferation and phosphorylation of p44/42 MAPK. However, Akt was not phosphorylated. Blocking the p44/42 MAPK pathway by using a specific mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitor (U0126) completely neutralized the effects of FGF7 and FGF10 on AM-1 cell proliferation. However, Anti FGF7 and FGF10 neutralizing antibodies did not decrease cell proliferation and MAPK phosphorylation of AM-1 cells. These results suggested that FGF7 and FGF10 are involved in the proliferation of ameloblastoma cells through the MAPK pathway.
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November 2013
Volume 43 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Nakao, Y., Mitsuyasu, T., Kawano, S., Nakamura, N., Kanda, S., & Nakamura, S. (2013). Fibroblast growth factors 7 and 10 are involved in ameloblastoma proliferation via the mitogen-activated protein kinase pathway. International Journal of Oncology, 43, 1377-1384. https://doi.org/10.3892/ijo.2013.2081
MLA
Nakao, Y., Mitsuyasu, T., Kawano, S., Nakamura, N., Kanda, S., Nakamura, S."Fibroblast growth factors 7 and 10 are involved in ameloblastoma proliferation via the mitogen-activated protein kinase pathway". International Journal of Oncology 43.5 (2013): 1377-1384.
Chicago
Nakao, Y., Mitsuyasu, T., Kawano, S., Nakamura, N., Kanda, S., Nakamura, S."Fibroblast growth factors 7 and 10 are involved in ameloblastoma proliferation via the mitogen-activated protein kinase pathway". International Journal of Oncology 43, no. 5 (2013): 1377-1384. https://doi.org/10.3892/ijo.2013.2081