Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer

  • Authors:
    • Changyuan Hu
    • Feng Lin
    • Guangbao Zhu
    • Xiangyang Xue
    • Yujie Ding
    • Zhiguang Zhao
    • Lifang Zhang
    • Xian Shen
  • View Affiliations

  • Published online on: August 23, 2013     https://doi.org/10.3892/ijo.2013.2078
  • Pages: 1487-1494
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

CXCL14, a new member of the CXC subfamily of chemokines, is differentially expressed in several types of tumors. The expression of CXCL14 and its clinical significance in gastric cancer are unclear to date. In this study, the expression of CXCL14 was detected by quantitative PCR and immunohistochemistry assay. DNA methylation was analyzed by bisulfite sequencing PCR. Student's t-test and Kruskal-Wallis H test were used to evaluate the differences of the CXCL14 expression between the groups. Kaplan-Meier survival curve and Cox regression model were used to evaluate the clinical significance of CXCL14 expression in gastric cancer. Data indicated that the levels of CXCL14 mRNA declined (P<0.001) in gastric carcinoma tissues compared to the paired normal tissues. Immunohistochemical analysis also showed the decrease of CXCL14 protein in the tumor tissue (P<0.001). Analysis of CpG islands methylation in CXCL14 promoter region and first exon area indicated that the abnormal hypermethylation of promoter region in tumor tissue is one of the mechanisms causing the reduction. When gastric cancer cells were demethylated with 5-Aza-2'-deoxycytidine, CXCL14 expression was restored. Downregulation of CXCL14 was associated with the depth of penetration (P<0.001) and positively correlated with prognosis in stage III/IV (P=0.046). In conclusion, it is possible that CXCL14 is involved in the development and progression of gastric cancer. Hypermethylation in the promoter is one of the reasons that CXCL14 has lower expression in gastric adenocarcinoma tissues. The level of CXCL14 expression may be a valuable adjuvant parameter in predicting the prognosis of gastric cancer patients and, thus, a potential therapeutic target.
View Figures
View References

Related Articles

Journal Cover

November 2013
Volume 43 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Hu, C., Lin, F., Zhu, G., Xue, X., Ding, Y., Zhao, Z. ... Shen, X. (2013). Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer. International Journal of Oncology, 43, 1487-1494. https://doi.org/10.3892/ijo.2013.2078
MLA
Hu, C., Lin, F., Zhu, G., Xue, X., Ding, Y., Zhao, Z., Zhang, L., Shen, X."Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer". International Journal of Oncology 43.5 (2013): 1487-1494.
Chicago
Hu, C., Lin, F., Zhu, G., Xue, X., Ding, Y., Zhao, Z., Zhang, L., Shen, X."Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer". International Journal of Oncology 43, no. 5 (2013): 1487-1494. https://doi.org/10.3892/ijo.2013.2078