8-bromo-5-hydroxy-7-methoxychrysin targeting for inhibition of the properties of liver cancer stem cells by modulation of Twist signaling

  • Authors:
    • Kai-Qun Ren
    • Xiao-Zheng Cao
    • Zhi-Hong Liu
    • Hui Guo
    • Mei-Fang Quan
    • Fei Liu
    • Ling Jiang
    • Hong-Lin Xiang
    • Xi-Yun Deng
    • Jian-Guo Cao
  • View Affiliations

  • Published online on: August 21, 2013     https://doi.org/10.3892/ijo.2013.2071
  • Pages: 1719-1729
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Emerging evidence has suggested that cancer stem cells with expression of surface biomarkers including CD133 and CD44 have more aggressive biological behavior, including epithelial-mesenchymal transition (EMT), which are closely related to invasion. The upregulation and nuclear relocation of the EMT regulator Twist1 have been implicated in the tumor invasion and metastasis of human hepatocellular carcinoma (HCC). In this study, we aimed to isolate and characterize a small population of CD133+ cells that existed in the HCC cell line SMMC-7721 by MACS and investigated the possible roles of 8-bromo-7-methoxychrysin (BrMC), a synthetic analogue of chrysin, in inhibiting the properties of CD133+ sphere-forming cells (SFCs) derived from the HCC cell line SMMC-7721, namely liver cancer stem cells (LCSCs). Based on the data, BrMC inhibited the proliferation, self-renewal and invasion of LCSCs in vitro and in vivo, downregulated the expression of the LCSC biomarkers CD133 and CD44 and induced EMT by downregulating the expression of Twist and β-catenin in LCSCs. BrMC potentiated the inhibition of LCSCs self-renewal after reduction of twist protein levels, which was attenuated when twist was overexpressed. This study not only provides an important experimental and theoretical basis for investigation of BrMC in LCSCs, but also helps in the development of effective therapeutic medicine for HCC.
View Figures
View References

Related Articles

Journal Cover

November 2013
Volume 43 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Ren, K., Cao, X., Liu, Z., Guo, H., Quan, M., Liu, F. ... Cao, J. (2013). 8-bromo-5-hydroxy-7-methoxychrysin targeting for inhibition of the properties of liver cancer stem cells by modulation of Twist signaling. International Journal of Oncology, 43, 1719-1729. https://doi.org/10.3892/ijo.2013.2071
MLA
Ren, K., Cao, X., Liu, Z., Guo, H., Quan, M., Liu, F., Jiang, L., Xiang, H., Deng, X., Cao, J."8-bromo-5-hydroxy-7-methoxychrysin targeting for inhibition of the properties of liver cancer stem cells by modulation of Twist signaling". International Journal of Oncology 43.5 (2013): 1719-1729.
Chicago
Ren, K., Cao, X., Liu, Z., Guo, H., Quan, M., Liu, F., Jiang, L., Xiang, H., Deng, X., Cao, J."8-bromo-5-hydroxy-7-methoxychrysin targeting for inhibition of the properties of liver cancer stem cells by modulation of Twist signaling". International Journal of Oncology 43, no. 5 (2013): 1719-1729. https://doi.org/10.3892/ijo.2013.2071