Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets

  • Authors:
    • Wei Zhang
    • Hui Chen
    • Dian-Lei Liu
    • Hong Li
    • Jiang Luo
    • Jian-Hong Zhang
    • Ye Li
    • Kang-Jie Chen
    • Hong-Fei Tong
    • Sheng-Zhang Lin
  • View Affiliations

  • Published online on: February 22, 2013     https://doi.org/10.3892/ijo.2013.1839
  • Pages: 1189-1196
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Abstract

The aim of this study was to evaluate whether emodin can overcome the chemoresistance of the gemcitabine-resistant cancer cell line (Bxpc-3/Gem) in vitro. The cell line Bxpc-3/Gem was derived from the human pancreatic cancer cell line Bxpc-3. We found that Bxpc-3/Gem cells were characterized by a series of morphological changes with a resistance index of 43.51 comparing with the parental cell line. Emodin reduced Bxpc-3/Gem cell proliferation in a dose-dependent manner. Emodin and gemcitabine combination treatments resulted in decreased cell proliferation and increased apoptosis in Bxpc-3/Gem cells. In addition, combination treatments resulted in downregulation of gene and protein expression of MDR-1 (P-gp), NF-κB, XIAP, survivin, as well as inhibition of NF-κB activity and P-gp function. These observations suggest that emodin may sensitize the pancreatic cancer gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine therapy via inhibition of survival signaling.
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April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J. ... Lin, S. (2013). Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets. International Journal of Oncology, 42, 1189-1196. https://doi.org/10.3892/ijo.2013.1839
MLA
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J., Li, Y., Chen, K., Tong, H., Lin, S."Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets". International Journal of Oncology 42.4 (2013): 1189-1196.
Chicago
Zhang, W., Chen, H., Liu, D., Li, H., Luo, J., Zhang, J., Li, Y., Chen, K., Tong, H., Lin, S."Emodin sensitizes the gemcitabine-resistant cell line Bxpc-3/Gem to gemcitabine via downregulation of NF-κB and its regulated targets". International Journal of Oncology 42, no. 4 (2013): 1189-1196. https://doi.org/10.3892/ijo.2013.1839