Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses

  • Authors:
    • Zhi-Liang Duan
    • Zhi-Bin Wang
    • Jiang-Long Guo
    • Wen-Quan Liu
    • Jun Hu
    • Jing Li
    • Si-Na Wang
    • Qiang Li
    • Jin-Sheng Wen
  • View Affiliations

  • Published online on: February 20, 2013     https://doi.org/10.3892/ijo.2013.1834
  • Pages: 1482-1492
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Squamous cell carcinoma antigen (SCCA) is overexpressed in many squamous cell cancers and SCCA‑derived peptide-specific CD8+ cytotoxic T lymphocytes can display cytotoxicity against tumor cells. In the present study, we screened the SCCA amino acid sequence for potential HLA-A*0201-binding CD8+ T‑cell epitopes using two predictive computational algorithms. Seven epitope candidates were selected of which SCCA246-254(llpneidgl), SCCA223-231(sledvqakv), SCCA328‑336(vlhkafvev) and SCCA324‑332(vlsgvlhka) significantly stabilized HLA-A*0201 molecules on T2 cells. Both SCCA328‑336 and SCCA324-332 induced CD8+ IFN-γ+ T‑cell responses in HLA-A*0201-positive peripheral blood mononuclear cells as assessed by intracellular cytokine staining. Consistent with this, immunization with either SCCA328-336 or SCCA324‑332 effectively elicited CD8+ IFN-γ+ T cells in HLA-A*0201 transgenic mice as visualized by IFN-γ ELISPOT assay and intracellular cytokine staining. Furthermore, CD8+ T cells induced in vitro or in vivo by SCCA328-336 or SCCA324-332 demonstrated in vitro cytotoxicity against peptide-pulsed T2 cells and splenocytes, respectively. These novel SCCA‑derived CD8+ T‑cell epitopes described, herein, may be potentially important components for diagnostic reagents and immunotherapeutic vaccines for the treatment of squamous cell carcinomas.

Related Articles

Journal Cover

April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Duan, Z., Wang, Z., Guo, J., Liu, W., Hu, J., Li, J. ... Wen, J. (2013). Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses. International Journal of Oncology, 42, 1482-1492. https://doi.org/10.3892/ijo.2013.1834
MLA
Duan, Z., Wang, Z., Guo, J., Liu, W., Hu, J., Li, J., Wang, S., Li, Q., Wen, J."Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses". International Journal of Oncology 42.4 (2013): 1482-1492.
Chicago
Duan, Z., Wang, Z., Guo, J., Liu, W., Hu, J., Li, J., Wang, S., Li, Q., Wen, J."Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses". International Journal of Oncology 42, no. 4 (2013): 1482-1492. https://doi.org/10.3892/ijo.2013.1834