Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells

  • Authors:
    • Ying Bai
    • Guang-Rong Qiu
    • Fan Zhou
    • Li-Ying Gong
    • Feng Gao
    • Kai-Lai Sun
  • View Affiliations

  • Published online on: February 19, 2013     https://doi.org/10.3892/ijo.2013.1831
  • Pages: 1317-1324
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Abstract

Dicer, a member of the RNase III family, is the key enzyme required for the biogenesis of microRNAs and small interfering RNAs. Recent evidence indicates that DICER1 expression levels vary among different solid tumors and decreased or increased DICER1 expression has been associated with aggressive cancers. In this study, we assessed DICER1 expression levels in acute myeloid leukemia (AML) and investigated its biological effects and transcriptional regulation in leukemia cell lines. We demonstrated that DICER1 was overexpressed in AML patients and leukemia cell lines by real-time quantitative PCR and western blot analysis. A functional assay demonstrated that the silencing of DICER1 inhibited cell proliferation and promoted apoptosis in leukemia cell lines. We also demonstrated that DICER1 was upregulated by the hematopoietic transcription factor, GATA1, through luciferase, electrophoretic mobility shift and chromatin immunoprecipitation assays. These data suggest that DICER1 plays an important role in AML and the finding that the upregulation of DICER1 is induced by GATA1 may provide a framework for the understanding of differential DICER1 expression levels in multiple types of cancer.
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April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., & Sun, K. (2013). Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells . International Journal of Oncology, 42, 1317-1324. https://doi.org/10.3892/ijo.2013.1831
MLA
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., Sun, K."Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells ". International Journal of Oncology 42.4 (2013): 1317-1324.
Chicago
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., Sun, K."Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells ". International Journal of Oncology 42, no. 4 (2013): 1317-1324. https://doi.org/10.3892/ijo.2013.1831