Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways

  • Authors:
    • Jinshun Zhao
    • Linda Bowman
    • Ruth Magaye
    • Stephen S. Leonard
    • Vincent Castranova
    • Min Ding
  • View Affiliations

  • Published online on: February 15, 2013     https://doi.org/10.3892/ijo.2013.1828
  • Pages: 1349-1359
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Abstract

In this study, apoptosis and related signaling induced by WC-Co nanoparticles were investigated in JB6 cells and rat lung macrophages. Electron spin resonance (ESR) and fluorescent staining indicated that both WC-Co nanoparticles and fine particles stimulated reactive oxygen species (ROS) generation. Catalase exhibited an inhibitory effect on WC-Co nanoparticle-induced ROS as well as mitochondrial membrane permeability damage. Further study indicated that WC-Co nanoparticles elicited higher cytotoxicity and apoptotic induction than fine particles. Western blot analysis showed activation of proapoptotic factors including Fas, Fas-associated protein with death domain (FADD), caspase 3, 8 and 9, BID and BAX. In addition, both cytochrome c and apoptosis-inducing factor (AIF) were upregulated and released from mitochondria to the cytoplasm. Our findings demonstrate that, on a mass basis, WC-Co nanoparticles exhibit higher cytotoxicity and apoptotic induction than fine particles. Apoptosis induced by WC-Co nanoparticles and fine particles involves both extrinsic and intrinsic apoptosis pathways.
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April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zhao, J., Bowman, L., Magaye, R., Leonard, S.S., Castranova, V., & Ding, M. (2013). Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways. International Journal of Oncology, 42, 1349-1359. https://doi.org/10.3892/ijo.2013.1828
MLA
Zhao, J., Bowman, L., Magaye, R., Leonard, S. S., Castranova, V., Ding, M."Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways". International Journal of Oncology 42.4 (2013): 1349-1359.
Chicago
Zhao, J., Bowman, L., Magaye, R., Leonard, S. S., Castranova, V., Ding, M."Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways". International Journal of Oncology 42, no. 4 (2013): 1349-1359. https://doi.org/10.3892/ijo.2013.1828