The CD133+ subpopulation of the SW982 human synovial sarcoma cell line exhibits cancer stem-like characteristics

  • Authors:
    • Aiguo Liu
    • Baohua Feng
    • Wenguang Gu
    • Xiangyang Cheng
    • Tiejun Tong
    • Hongzhi Zhang
    • Yongzhen Hu
  • View Affiliations

  • Published online on: February 15, 2013     https://doi.org/10.3892/ijo.2013.1826
  • Pages: 1399-1407
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Abstract

Several soft tissues sarcomas have been reported to contain cancer stem-like cells (CSCs) or tumor-initiating cells, based on their ability to initiate and sustain tumor growth. However, these cells have not yet been identified in the human synovial sarcoma cell line SW982. CD133, a surface glycoprotein specific to stem and progenitor cells, has been described as a CSC marker in different tumor types. In the present study, we identified a CSC subpopulation in SW982 cells using the CD133 cell surface marker. CD133-positive (CD133+) cells were identified in SW982 cells (8.59%); these cells showed an increased ability to form spherical colonies and could self-renew in serum-starved culture conditions, compared to CD133-negative (CD133-) cells. Real-time PCR analysis of stemness genes revealed that the CD133+ subpopulation expresses higher levels of Bmi1, c-Myc, Nanog, Oct3/4 and Sox2. CD133+ cells showed increased resistance to cisplatin (CDDP) and doxorubicin (DXR), possibly due to upregulation of the ABCG2 drug transporter gene. In vivo studies revealed that the CD133+ subpopulation is highly tumorigenic. These findings indicate that CD133+ SW982 cells have characteristics similar to CSCs. This discovery may lead to the development of novel therapies that specifically target CD133+ synovial sarcoma CSCs.
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April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Liu, A., Feng, B., Gu, W., Cheng, X., Tong, T., Zhang, H., & Hu, Y. (2013). The CD133+ subpopulation of the SW982 human synovial sarcoma cell line exhibits cancer stem-like characteristics. International Journal of Oncology, 42, 1399-1407. https://doi.org/10.3892/ijo.2013.1826
MLA
Liu, A., Feng, B., Gu, W., Cheng, X., Tong, T., Zhang, H., Hu, Y."The CD133+ subpopulation of the SW982 human synovial sarcoma cell line exhibits cancer stem-like characteristics". International Journal of Oncology 42.4 (2013): 1399-1407.
Chicago
Liu, A., Feng, B., Gu, W., Cheng, X., Tong, T., Zhang, H., Hu, Y."The CD133+ subpopulation of the SW982 human synovial sarcoma cell line exhibits cancer stem-like characteristics". International Journal of Oncology 42, no. 4 (2013): 1399-1407. https://doi.org/10.3892/ijo.2013.1826