Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells

  • Authors:
    • Petr Vaňhara
    • Peter Horak
    • Dietmar Pils
    • Mariam Anees
    • Michaela Petz
    • Wolfgang Gregor
    • Robert Zeillinger
    • Michael Krainer
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  • Published online on: February 12, 2013     https://doi.org/10.3892/ijo.2013.1824
  • Pages: 1383-1389
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Abstract

Consequences of deregulated protein N-glycosylation on cancer pathogenesis are poorly understood. TUSC3 is a gene with a putative function in N-glycosylation, located on the short arm of chromosome 8. This is a chromosomal region of frequent genetic loss in ovarian cancer. We established recently that the expression of TUSC3 is epigenetically decreased in epithelial ovarian cancer compared to benign controls and provides prognostic information on patient survival. Therefore, we analyzed the consequences of silenced TUSC3 expression on proliferation, invasion and migration of ovarian cell lines. In addition, we performed subcellular fractionation, co-immunofluorescence and co-immunopre­cipitation experiments to establish the molecular localization of TUSC3 in ovarian cancer cells. We demonstrated that TUSC3 is localized in the endoplasmic reticulum as a subunit of the oligosaccharyltransferase complex and is capable of modulation of glycosylation patterning of ovarian cancer cells. Most importantly, silencing of TUSC3 enhances proliferation and migration of ovarian cancer cells in vitro. Our observations suggest a role for N-glycosylating events in ovarian cancer pathogenesis in general, and identify TUSC3 as a tumor suppressor gene in ovarian cancer in particular.
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April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Vaňhara, P., Horak, P., Pils, D., Anees, M., Petz, M., Gregor, W. ... Krainer, M. (2013). Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells. International Journal of Oncology, 42, 1383-1389. https://doi.org/10.3892/ijo.2013.1824
MLA
Vaňhara, P., Horak, P., Pils, D., Anees, M., Petz, M., Gregor, W., Zeillinger, R., Krainer, M."Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells". International Journal of Oncology 42.4 (2013): 1383-1389.
Chicago
Vaňhara, P., Horak, P., Pils, D., Anees, M., Petz, M., Gregor, W., Zeillinger, R., Krainer, M."Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells". International Journal of Oncology 42, no. 4 (2013): 1383-1389. https://doi.org/10.3892/ijo.2013.1824