Short hairpin RNA targeting FOXQ1 inhibits invasion and metastasis via the reversal of epithelial-mesenchymal transition in bladder cancer

  • Authors:
    • Zhaohui Zhu
    • Zhineng Zhu
    • Zili Pang
    • Yifei Xing
    • Feng Wan
    • Dongyang Lan
    • Haipeng Wang
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  • Published online on: February 5, 2013     https://doi.org/10.3892/ijo.2013.1807
  • Pages: 1271-1278
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Abstract

The epithelial-mesenchymal transition (EMT) promotes cancer invasion and metastasis, however, the integrative mechanisms that coordinate the process are incompletely understood. In this study, we defined a pivotal functional role for the Forkhead transcription factor FOXQ1 in regulating EMT in bladder cancer. We initially investigated the expression of FOXQ1, TGF-β1 and EMT biomarkers E-cadherin, Vimentin in 65 cases of bladder transitional cell carcinoma (BTCC) specimens by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunohistochemistry. Search results indicated that FOXQ1 expression was inversely correlated to E-cadherin, but positively to TGF-β1 and Vimentin in patients with BTCC (P<0.05). Furthermore, we aimed to construct short hairpin RNA (shRNA) expression plasmids against the FOXQ1 gene and transfect shRNAs into high metastatic potential human bladder cancer T24 cells with Lipofectamine 2000. RNAi-mediated suppression of FOXQ1 expression reversed the EMT process accompanied by upregulation of E-cadherin, as well as a loss expression of Vimentin in highly invasive T24 cells (P<0.05). The inhibition of FOXQ1 expression with shRNA vector also led T24 cells to acquire an epithelial cobblestone phenotype, significantly reduced motility and subsequent invasiveness of bladder cancer cells (P<0.05). In conclusion that FOXQ1 may be a novel EMT-inducing transcription factor through controlling the expression of E-cadherin and aggressiveness of cancer cells and targeting the transcription factor FOXQ1 could hence serve as a novel therapeutic strategy for cancer patients.
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April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zhu, Z., Zhu, Z., Pang, Z., Xing, Y., Wan, F., Lan, D., & Wang, H. (2013). Short hairpin RNA targeting FOXQ1 inhibits invasion and metastasis via the reversal of epithelial-mesenchymal transition in bladder cancer. International Journal of Oncology, 42, 1271-1278. https://doi.org/10.3892/ijo.2013.1807
MLA
Zhu, Z., Zhu, Z., Pang, Z., Xing, Y., Wan, F., Lan, D., Wang, H."Short hairpin RNA targeting FOXQ1 inhibits invasion and metastasis via the reversal of epithelial-mesenchymal transition in bladder cancer". International Journal of Oncology 42.4 (2013): 1271-1278.
Chicago
Zhu, Z., Zhu, Z., Pang, Z., Xing, Y., Wan, F., Lan, D., Wang, H."Short hairpin RNA targeting FOXQ1 inhibits invasion and metastasis via the reversal of epithelial-mesenchymal transition in bladder cancer". International Journal of Oncology 42, no. 4 (2013): 1271-1278. https://doi.org/10.3892/ijo.2013.1807