Identification of candidate biomarkers using the Experion™ automated electrophoresis system in serum samples from ovarian cancer patients

  • Authors:
    • Ju Hee Kim
    • Yong-Wan Kim
    • In-Wook Kim
    • Dong Chun Park
    • Yong Wook Kim
    • Keun-Ho Lee
    • Chun Keun Jang
    • Woong Shick Ahn
  • View Affiliations

  • Published online on: January 29, 2013     https://doi.org/10.3892/ijo.2013.1803
  • Pages: 1257-1262
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Ovarian cancer is the most common cause of disease-related death in women globally. Detection of ovarian cancer using new biomarkers is necessary for early diagnosis. To date, there have been no obvious biomarkers for ovarian cancer detection in the incipient stage. In this study, we discovered potential diagnostic serological biomarkers for ovarian cancer using the Experion™ automated electrophoresis system. Sera from 14 healthy women and 84 ovarian cancer patients at stages I- IV were applied to the Experion to compare the protein expression levels. To examine the protein expression pattern of Experion data, proteins in the samples were resolved using 10 and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by silver staining. The candidate biomarkers elevated in ovarian cancer were purified and determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. α-2-macroglobulin (173.7 kDa), ceruloplasmin (147 kDa), inter-α-trypsin inhibitor family heavy chain-related protein (126 kDa), C-1 inhibitor (115.2 kDa) and hemoglobin α/β (14.4 kDa were overexpressed in the ovarian cancer sera. This study documents a novel way to measure ovarian cancer or cancer-related proteins for biomarkers using the Experion assay system, which should be easily adaptable for high-throughput diagnosis to establish databases of ovarian cancer for clinical applications.
View Figures
View References

Related Articles

Journal Cover

April 2013
Volume 42 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kim, J.H., Kim, Y., Kim, I., Park, D.C., Kim, Y.W., Lee, K. ... Ahn, W.S. (2013). Identification of candidate biomarkers using the Experion™ automated electrophoresis system in serum samples from ovarian cancer patients. International Journal of Oncology, 42, 1257-1262. https://doi.org/10.3892/ijo.2013.1803
MLA
Kim, J. H., Kim, Y., Kim, I., Park, D. C., Kim, Y. W., Lee, K., Jang, C. K., Ahn, W. S."Identification of candidate biomarkers using the Experion™ automated electrophoresis system in serum samples from ovarian cancer patients". International Journal of Oncology 42.4 (2013): 1257-1262.
Chicago
Kim, J. H., Kim, Y., Kim, I., Park, D. C., Kim, Y. W., Lee, K., Jang, C. K., Ahn, W. S."Identification of candidate biomarkers using the Experion™ automated electrophoresis system in serum samples from ovarian cancer patients". International Journal of Oncology 42, no. 4 (2013): 1257-1262. https://doi.org/10.3892/ijo.2013.1803