ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression

  • Authors:
    • Ji-Young Jang
    • Yoon-Kyung Jeon
    • Choong-Eun Lee
    • Chul-Woo Kim
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  • Published online on: December 13, 2012     https://doi.org/10.3892/ijo.2012.1736
  • Pages: 574-582
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Abstract

Suppressor of cytokine signaling 1 (SOCS1) is a negative regulator of Janus kinase and the signal transducer and activation of transcription (Jak-STAT) pathway. SOCS-1 is known to be silenced by aberrant promoter methylation in human hepatocellular carcinoma (HCC) during early tumorigenesis, therefore, a strategy to restore SOCS1 expression can be utilized for cancer therapy. Here, we examined the influence of adenine nucleotide translocase 2 (ANT2) suppression by short-hairpin RNA (shRNA) on SOCS1 expression and its downstream effect in HCC. ANT2 shRNA treatment led to restoration of SOCS1 expression along with its promoter demethylation in Hep3B cells, which was accompanied by decreased DNA methyltransferase 1 (DNMT1) activity through the suppression of Ras/PI3K/Akt signaling. Restoration of SOCS1 by ANT2 knockdown, subsequently, inhibited STAT3 activity and downregulated the expression of miR-21, which has been reported to be an important onco-miR in HCC. Downregulation of miR-21 efficiently suppressed Hep3B cell proliferation in vitro with a comparable level to ANT2 shRNA treatment. ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression.
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February 2013
Volume 42 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Jang, J., Jeon, Y., Lee, C., & Kim, C. (2013). ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression. International Journal of Oncology, 42, 574-582. https://doi.org/10.3892/ijo.2012.1736
MLA
Jang, J., Jeon, Y., Lee, C., Kim, C."ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression". International Journal of Oncology 42.2 (2013): 574-582.
Chicago
Jang, J., Jeon, Y., Lee, C., Kim, C."ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression". International Journal of Oncology 42, no. 2 (2013): 574-582. https://doi.org/10.3892/ijo.2012.1736