Open Access

Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells

  • Authors:
    • Yong-Sheng Jiang
    • Masayo Maeda
    • Mayumi Okamoto
    • Mikiko Fujii
    • Ryuichiro Fukutomi
    • Masato Hori
    • Masaaki Tatsuka
    • Takahide Ota
  • View Affiliations

  • Published online on: December 7, 2012     https://doi.org/10.3892/ijo.2012.1730
  • Pages: 460-468
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Rho GDP-dissociation inhibitors (RhoGDIs) are regulators of Rho family GTPases. RhoGDIβ has been implicated in cancer progression, but its precise role remains unclear. We determined the subcellular localization of RhoGDIβ and examined the effects of its overexpression and RNAi knockdown in cancer cells. Immunofluorescence staining showed that RhoGDIβ localized to centrosomes in human cancer cells. In HeLa cells, exogenous GFP-tagged RhoGDIβ localized to centrosomes and its overexpression caused prolonged mitosis and aberrant cytokinesis in which the cell shape was distorted. RNAi knockdown of RhoGDIβ led to increased incidence of monopolar spindle mitosis resulting in polyploid cells. These results suggest that RhoGDIβ has mitotic functions, including regulation of cytokinesis and bipolar spindle formation. The dysregulated expression of RhoGDIβ may contribute to cancer progression by disrupting these processes.
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February 2013
Volume 42 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Jiang, Y., Maeda, M., Okamoto, M., Fujii, M., Fukutomi, R., Hori, M. ... Ota, T. (2013). Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells. International Journal of Oncology, 42, 460-468. https://doi.org/10.3892/ijo.2012.1730
MLA
Jiang, Y., Maeda, M., Okamoto, M., Fujii, M., Fukutomi, R., Hori, M., Tatsuka, M., Ota, T."Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells". International Journal of Oncology 42.2 (2013): 460-468.
Chicago
Jiang, Y., Maeda, M., Okamoto, M., Fujii, M., Fukutomi, R., Hori, M., Tatsuka, M., Ota, T."Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells". International Journal of Oncology 42, no. 2 (2013): 460-468. https://doi.org/10.3892/ijo.2012.1730