Open Access

Fibulin-3 promoter methylation alters the invasive behavior of non-small cell lung cancer cell lines via MMP-7 and MMP-2 regulation

  • Authors:
    • Eun Jin Kim
    • So Yong Lee
    • Mi Kyung Woo
    • Soo Im Choi
    • Tae Rim Kim
    • Min-Jung Kim
    • Kug Chan Kim
    • Eun Wie Cho
    • In Gyu Kim
  • View Affiliations

  • Published online on: September 7, 2011     https://doi.org/10.3892/ijo.2011.1191
  • Pages: 402-408
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Abstract

Fibulin-3, an extracellular glycoprotein, has been suggested as having functions in tissue regeneration and organogenesis. However, its role in cancer remains unclear. We show here that fibulin-3 was silenced by hypermethylation of the promoter region in the relatively invasive A549 non-small cell lung cancer (NSCLC) cells compared with less invasive H460 NSCLC cells. Enforced expression of fibulin-3 in A549 cells down-regulated cellular MMP-7 and MMP-2, which was followed by inhibition of cell invasiveness. Conversely, suppression of fibulin-3 expression with siRNA in H460 cells showed the opposite effect. These results indicate that fibulin-3 is a negative regulator of invasiveness in NSCLC and further studies are needed for its therapeutic applications in treatment of NSCLC.

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February 2012
Volume 40 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kim, E.J., Lee, S.Y., Woo, M.K., Choi, S.I., Kim, T.R., Kim, M. ... Kim, I.G. (2012). Fibulin-3 promoter methylation alters the invasive behavior of non-small cell lung cancer cell lines via MMP-7 and MMP-2 regulation. International Journal of Oncology, 40, 402-408. https://doi.org/10.3892/ijo.2011.1191
MLA
Kim, E. J., Lee, S. Y., Woo, M. K., Choi, S. I., Kim, T. R., Kim, M., Kim, K. C., Cho, E. W., Kim, I. G."Fibulin-3 promoter methylation alters the invasive behavior of non-small cell lung cancer cell lines via MMP-7 and MMP-2 regulation". International Journal of Oncology 40.2 (2012): 402-408.
Chicago
Kim, E. J., Lee, S. Y., Woo, M. K., Choi, S. I., Kim, T. R., Kim, M., Kim, K. C., Cho, E. W., Kim, I. G."Fibulin-3 promoter methylation alters the invasive behavior of non-small cell lung cancer cell lines via MMP-7 and MMP-2 regulation". International Journal of Oncology 40, no. 2 (2012): 402-408. https://doi.org/10.3892/ijo.2011.1191