Deciphering the role of paclitaxel in the SKGT4 human esophageal adenocarcinoma cell line

  • Authors:
    • Seo Ju Kim
    • Myoung Ja Chung
    • Jong-Suk Kim
    • Bumseok Kim
    • Woo Hyun Park
    • Suhn Hee Kim
    • Sung Zoo Kim
    • Ju-Seog Lee
    • Soo Mi Kim
  • View Affiliations

  • Published online on: July 20, 2011     https://doi.org/10.3892/ijo.2011.1135
  • Pages: 1587-1591
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Abstract

Paclitaxel (taxol) has been used for the treatment of various human tumors and is an exceedingly efficient chemotherapy agent against esophageal cancer. However, the precise molecular mechanisms of paclitaxel effects on human esophageal adenocarcinoma cells are not well understood. MTT assay and cell cycle analysis were performed to examine the mechanism of antiproliferative and cell viability effects of paclitaxel in human esophageal adenocarcinoma cancer cells. Western blotting was also used to examine the cell cycle- and apoptosis-related proteins. Paclitaxel inhibited the proliferation of SKGT4 cells in a dose- and time-dependent manner with G2/M arrest. In addition, paclitaxel induced apoptosis through the activation of caspase-3 followed by PARP degradation. In conclusion, our results suggest that paclitaxel leads to mitotic cell cycle arrest following G2/M arrest and induces apoptosis via a caspase-3 pathway in SKGT4 cells.

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December 2011
Volume 39 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kim, S.J., Chung, M. ., Kim, J., Kim, B., Park, W.H., Kim, S.H. ... Kim, S.M. (2011). Deciphering the role of paclitaxel in the SKGT4 human esophageal adenocarcinoma cell line. International Journal of Oncology, 39, 1587-1591. https://doi.org/10.3892/ijo.2011.1135
MLA
Kim, S. J., Chung, M. ., Kim, J., Kim, B., Park, W. H., Kim, S. H., Kim, S. Z., Lee, J., Kim, S. M."Deciphering the role of paclitaxel in the SKGT4 human esophageal adenocarcinoma cell line". International Journal of Oncology 39.6 (2011): 1587-1591.
Chicago
Kim, S. J., Chung, M. ., Kim, J., Kim, B., Park, W. H., Kim, S. H., Kim, S. Z., Lee, J., Kim, S. M."Deciphering the role of paclitaxel in the SKGT4 human esophageal adenocarcinoma cell line". International Journal of Oncology 39, no. 6 (2011): 1587-1591. https://doi.org/10.3892/ijo.2011.1135