The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-κB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells

  • Authors:
    • S. Moalic-Juge
    • B. Liagre
    • R. Duval
    • C. Corbiere
    • A. Bianchi
    • K. Bordji
    • C. Bosgiraud
    • J.-L. Beneytout
  • View Affiliations

  • Published online on: June 1, 2002     https://doi.org/10.3892/ijo.20.6.1255
  • Pages: 1255-1262
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Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) have been shown to produce an anti-proliferative and pro-apoptotic effect on different types of cancer cell lines. Previously, we demonstrated that high dose of NS-398 (100 μM), a selective cyclooxygenase-2 inhibitor, induced a cell cycle slowing or arrest and, in contrast to low dose (10 μM), a marked decrease in apoptosis in human 1547 osteosarcoma cells. In this study, we investigated particularly the effect of 100 μM NS-398 on p53 and p21 expression, caspase activities and nuclear factor-κB (NF-κB). We found a correlation between p53, p21 mRNA expression and NF-κB activation and, we observed an induction of heat shock protein 70 expression with a large decrease in caspase-3 activity after 100 μM NS-398 treatment. Moreover, the inhibition of apoptosis was correlated with an increase in bcl-2/bax ratio. Our new findings confirm the novel anti-apoptotic property of NS-398 at 100 μM, as we previously found, which contrasts to the described NS-398 pro-apoptotic effect on other cancer cell lines.

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June 2002
Volume 20 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Moalic-Juge, S., Liagre, B., Duval, R., Corbiere, C., Bianchi, A., Bordji, K. ... Beneytout, J. (2002). The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-κB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells. International Journal of Oncology, 20, 1255-1262. https://doi.org/10.3892/ijo.20.6.1255
MLA
Moalic-Juge, S., Liagre, B., Duval, R., Corbiere, C., Bianchi, A., Bordji, K., Bosgiraud, C., Beneytout, J."The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-κB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells". International Journal of Oncology 20.6 (2002): 1255-1262.
Chicago
Moalic-Juge, S., Liagre, B., Duval, R., Corbiere, C., Bianchi, A., Bordji, K., Bosgiraud, C., Beneytout, J."The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-κB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells". International Journal of Oncology 20, no. 6 (2002): 1255-1262. https://doi.org/10.3892/ijo.20.6.1255