The rgl-1 is a legitimate homologue of lethal giant larvae recessive oncogene in rat

  • Authors:
    • Y.-S. Kim
    • K.-H. Baek
    • K. Y. Lee
    • H.-M. Chung
    • K.-A. Lee
    • J. J. Ko
    • K. Y. Cha
  • View Affiliations

  • Published online on: June 1, 2002     https://doi.org/10.3892/ijo.20.6.1219
  • Pages: 1219-1225
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Abstract

We have cloned a rat homologue of the Drosophila recessive oncogene lethal (2) giant larvae from rat brain by RT-PCR using primers prepared from sequences conserved amongst lgl family genes. Sequence analysis predicts that the rat rgl-1 gene encodes a 1,036 amino acid polypeptide with a molecular weight of approximately 112 kDa, which contains a domain characteristic of WD-40 proteins. Northern blot analysis revealed that the highest expression of rgl-1 is detected in the testis, with moderate expression in ovary, brain, spleen, and kidney. Since there is a high degree of amino acid similarity among lgl proteins in various species, it is likely that there is an evolutionary relationship among these proteins. The amino acid identity of rgl-1 to Drosophila l(2)gl and mouse mgl-1 proteins showed 30.6 and 96.8%, respectively. The rat tomosyn, previously known as a homologue of Drosophila l(2)gl, showed much lower amino acid identity to Drosophila l(2)gl and mouse mgl-1 proteins (17.8 and 20%, respectively). Functional analysis showed that the expression of a rat rgl-1 cDNA in Saccharomyces cerevisiae missing sop genes, the yeast homologues of the Drosophila l(2)gl, restored partially the Na+ tolerance of the cell. Taken together, these results indicate that rgl-1, not tomosyn, is the legitimate homologue of lgl gene.

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June 2002
Volume 20 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kim, Y., Baek, K., Lee, K.Y., Chung, H., Lee, K., Ko, J.J., & Cha, K.Y. (2002). The rgl-1 is a legitimate homologue of lethal giant larvae recessive oncogene in rat. International Journal of Oncology, 20, 1219-1225. https://doi.org/10.3892/ijo.20.6.1219
MLA
Kim, Y., Baek, K., Lee, K. Y., Chung, H., Lee, K., Ko, J. J., Cha, K. Y."The rgl-1 is a legitimate homologue of lethal giant larvae recessive oncogene in rat". International Journal of Oncology 20.6 (2002): 1219-1225.
Chicago
Kim, Y., Baek, K., Lee, K. Y., Chung, H., Lee, K., Ko, J. J., Cha, K. Y."The rgl-1 is a legitimate homologue of lethal giant larvae recessive oncogene in rat". International Journal of Oncology 20, no. 6 (2002): 1219-1225. https://doi.org/10.3892/ijo.20.6.1219