The role of the proteasome in apoptosis induced by anthracycline anticancer agents

  • Authors:
    • Ken-Ichi Kiyomiya
    • Masaru Kurebe
    • Hiroshi Nakagawa
    • Saburou Matsuo
  • View Affiliations

  • Published online on: June 1, 2002     https://doi.org/10.3892/ijo.20.6.1205
  • Pages: 1205-1209
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Abstract

To elucidate the involvement of proteasome inhibition in apoptosis induced by anthracycline anticancer agents, we investigated the interaction between the proteasome and anthracycline anticancer agents, and the function of the proteasome in apoptosis. Exposure of L1210 mouse lymphocytic leukemia cells to adriamycin (ADM) or 4'-O-tetrahydropyranyladriamycin (THP) resulted in apoptosis in a dose-dependent manner: 5 μM ADM and 0.5 μM THP induced apoptosis efficiently, but the effects of 10 μM ADM and 5 μM THP were markedly decreased or completely absent. Carbobenzoxy-leucyl-leucyl-leucinal (Z-LLLal), a specific proteasome inhibitor, also induced dose-dependent apoptosis of the cells. The inhibitory effect of THP on chymotrypsin-like protease activity of proteasomes purified from the cytosol of L1210 cells was stronger than that of ADM. Both of these agents showed reversible non-competitive inhibitory effects. The intracellular content of ubiquitinated protein increased in ADM-, THP- or Z-LLLal-treated L1210 cells during apoptosis. These results suggested that anthracycline anticancer agents induce apoptosis by interacting, at least in part, with proteasomes.

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June 2002
Volume 20 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kiyomiya, K., Kurebe, M., Nakagawa, H., & Matsuo, S. (2002). The role of the proteasome in apoptosis induced by anthracycline anticancer agents. International Journal of Oncology, 20, 1205-1209. https://doi.org/10.3892/ijo.20.6.1205
MLA
Kiyomiya, K., Kurebe, M., Nakagawa, H., Matsuo, S."The role of the proteasome in apoptosis induced by anthracycline anticancer agents". International Journal of Oncology 20.6 (2002): 1205-1209.
Chicago
Kiyomiya, K., Kurebe, M., Nakagawa, H., Matsuo, S."The role of the proteasome in apoptosis induced by anthracycline anticancer agents". International Journal of Oncology 20, no. 6 (2002): 1205-1209. https://doi.org/10.3892/ijo.20.6.1205