Hepatocarcinoma cell lines down-regulate matrix metalloproteinase-2 expression in human hepatic myofibroblasts

  • Authors:
    • Jean-Frederic Blanc
    • Christele Bisson
    • Francis Frankenne
    • Agnes Noel
    • Carine Munaut
    • Alain Colige
    • Julien Collette
    • Jean Rosenbaum
    • Jean-Michel Foidart
  • View Affiliations

  • Published online on: June 1, 2002     https://doi.org/10.3892/ijo.20.6.1129
  • Pages: 1129-1136
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Abstract

Matrix metalloproteinases (MMPs) are a family of proteases that degrade extracellular matrix components and are involved in tumor progression and metastasis. We studied the pattern of expression of MMP-2 by human hepatoma cell lines and human hepatic myofibroblasts and its regulation in co-culture between these cell types. MMP-2 expression was studied by zymography and semi-quantitative RT-PCR. The expression of MT1-MMP (involved in MMP-2 activation) was studied by Western blot analysis and RT-PCR and the secretion of TIMP-2 by ELISA and RT-PCR. Myofibroblasts expressed high levels of MMP-2, MT1-MMP and TIMP-2. The hepatoma cell lines HepG2, HuH7 and Hep3B did not express MMP-2 and weakly expressed TIMP-2 and MT1-MMP. In co-culture, no modulation of MT1-MMP or TIMP-2 expression was observed. A strong decrease in myofibroblast MMP-2 expression was seen in the presence of hepatoma cell lines. This was partly reproduced by their conditioned media. We conclude that hepatoma cell lines down-regulate the expression of MMP-2 by myofibroblasts.

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June 2002
Volume 20 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Blanc, J., Bisson, C., Frankenne, F., Noel, A., Munaut, C., Colige, A. ... Foidart, J. (2002). Hepatocarcinoma cell lines down-regulate matrix metalloproteinase-2 expression in human hepatic myofibroblasts. International Journal of Oncology, 20, 1129-1136. https://doi.org/10.3892/ijo.20.6.1129
MLA
Blanc, J., Bisson, C., Frankenne, F., Noel, A., Munaut, C., Colige, A., Collette, J., Rosenbaum, J., Foidart, J."Hepatocarcinoma cell lines down-regulate matrix metalloproteinase-2 expression in human hepatic myofibroblasts". International Journal of Oncology 20.6 (2002): 1129-1136.
Chicago
Blanc, J., Bisson, C., Frankenne, F., Noel, A., Munaut, C., Colige, A., Collette, J., Rosenbaum, J., Foidart, J."Hepatocarcinoma cell lines down-regulate matrix metalloproteinase-2 expression in human hepatic myofibroblasts". International Journal of Oncology 20, no. 6 (2002): 1129-1136. https://doi.org/10.3892/ijo.20.6.1129