Reactivity of tumor-draining lymph nodes and the nitric oxide pathway

  • Authors:
    • L. Davel
    • A. M. Eijan
    • M. E. Sales
    • A. Espanol
    • A. D'Agostino
    • I. Piccardo
    • L. Lauria
    • E. S. De Lustig
    • M. A. Jasnis
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.59
  • Pages: 59-67
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Regional lymph nodes are important in the generation of tumor-directed immune responses. The relationship between nitric oxide synthase (NOS) expression and the biological behavior of tumor-draining lymph node (TDLNs) cells in vivo was determined using a spontaneously arising BALB/c mammary adenocarcinoma S13. We first demonstrated a reduction of tumor size and tumor-induced angiogenesis by blocking NOS activity in vivo. TDLNs harvested from tumor-bearing mice (TBM) on day 16 after tumor implant, showed enhanced NOS activity and NOS expression compared to control nodes. Identification of the NOS isoforms present in TDLNs resulted in expression of neuronal NOS (nNOS), endothelial NOS (eNOS) and absence of inducible NOS (iNOS). TDLN cells admixed with tumor cells and inoculated into normal mice (Winn assay) induced a reduction of tumor growth although, when inoculated alone, were able to induce the formation of new blood vessels (angiogenesis). Our data indicate that the in vivo antitumor activity of TDLN cells is modulated by a balance between angiogenesis and antitumor effectors. In our model, when trafficking of leukocytes is obviated, the control of tumor growth by TDLN cells can be explained in part by an antitumor activity great enough to exceed the angiogenic component elicited by the same cells, leading to a reduction of tumor size.

Related Articles

Journal Cover

January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Davel, L., Eijan, A.M., Sales, M.E., Espanol, A., D'Agostino, A., Piccardo, I. ... Jasnis, M.A. (2002). Reactivity of tumor-draining lymph nodes and the nitric oxide pathway. International Journal of Oncology, 20, 59-67. https://doi.org/10.3892/ijo.20.1.59
MLA
Davel, L., Eijan, A. M., Sales, M. E., Espanol, A., D'Agostino, A., Piccardo, I., Lauria, L., De Lustig, E. S., Jasnis, M. A."Reactivity of tumor-draining lymph nodes and the nitric oxide pathway". International Journal of Oncology 20.1 (2002): 59-67.
Chicago
Davel, L., Eijan, A. M., Sales, M. E., Espanol, A., D'Agostino, A., Piccardo, I., Lauria, L., De Lustig, E. S., Jasnis, M. A."Reactivity of tumor-draining lymph nodes and the nitric oxide pathway". International Journal of Oncology 20, no. 1 (2002): 59-67. https://doi.org/10.3892/ijo.20.1.59