Expression of metastasis-related genes in human epithelial ovarian tumors

  • Authors:
    • C. A. Herrera
    • L. Xu
    • C. D. Bucana
    • E. G. Silva
    • K. R. Hess
    • D. M. Gershenson
    • I. J. Fidler
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.5
  • Pages: 5-13
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Abstract

We examined the expression level of several genes that regulate distinct steps of metastasis in 55 formalin-fixed, paraffin-embedded, archival specimens of primary human ovarian carcinoma from patients undergoing curative surgery. The expression of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF), basic fibroblast growth factor (bFGF), E-cadherin, type IV collagenase, matrix metalloproteinase (MMP-2 and MMP-9), and interleukin 8 (IL-8) was examined by a colorimetric in situ mRNA hybridization technique. The expression level of E-cadherin, MMP-2, MMP-9, VEGF, and IL-8 mRNA correlated with disease stages. The ratio of type IV collagenase expression (mean of the expression of MMP-2 and MMP-9) to E-cadherin expression (MMP:E-cadherin ratio) increased with increasing stage of disease (p<0.0001). Death rates significantly increased with high MMP:E-cadherin ratio (p=0.0005). Multivariate analysis of overall survival showed that the MMP:E-cadherin ratio was a significant independent prognostic factor, even after adjustment for known prognostic factors, such as histology, stage, and age.

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January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Herrera, C.A., Xu, L., Bucana, C.D., Silva, E.G., Hess, K.R., Gershenson, D.M., & Fidler, I.J. (2002). Expression of metastasis-related genes in human epithelial ovarian tumors. International Journal of Oncology, 20, 5-13. https://doi.org/10.3892/ijo.20.1.5
MLA
Herrera, C. A., Xu, L., Bucana, C. D., Silva, E. G., Hess, K. R., Gershenson, D. M., Fidler, I. J."Expression of metastasis-related genes in human epithelial ovarian tumors". International Journal of Oncology 20.1 (2002): 5-13.
Chicago
Herrera, C. A., Xu, L., Bucana, C. D., Silva, E. G., Hess, K. R., Gershenson, D. M., Fidler, I. J."Expression of metastasis-related genes in human epithelial ovarian tumors". International Journal of Oncology 20, no. 1 (2002): 5-13. https://doi.org/10.3892/ijo.20.1.5