Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines

  • Authors:
    • F. Wu
    • M. Nishioka
    • J. Fujita
    • M. Murota
    • Y. Ohtsuki
    • T. Ishida
    • S. Kuriyama
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.31
  • Pages: 31-37
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Abstract

It has been reported that the cytokeratin 19 (CK19) fragment, CYFRA 21-1, is increased in sera of some hepatocellular carcinoma patients. We hypothesized that CYFRA 21-1 might be released directly from hepatocellular carcinoma. To investigate this mechanism, we evaluated expression of CK19 in 8 human cell lines by immunoradiometric assay, Western blotting, immunohistochemistry, and reverse transcriptase-polymerase chain reaction in Chang liver cells, 2 cholangiocarcinoma cell lines, and 5 hepatocellular carcinoma cell lines. Three of 5 hepatocellular carcinoma cell lines released CYFRA 21-1, synthesized CK19 protein and expressed mRNA for CK19, as observed in 2 cholangiocarcinoma cell lines. In contrast, there was no expression of CK19 in Chang liver cells or 2 of 5 hepatocellular carcinoma cell lines. Analysis of genomic DNA for CK19 demonstrated that exon 1 was not amplified in the 3 cell lines not expressing CK19. However, there were no point mutations within exon 1 and the promoter region of CK19 in hepatocellular carcinoma cell lines. Our present study demonstrates that: i) the expression of CK19 was evident in some human hepatocellular carcinoma cell lines, and ii) the expression of mRNA for CK19 was related to the release of CYFRA 21-1. These results partially clarify the mechanism by which some hepatocellular carcinoma cell lines produce CK19 and others do not.

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January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Wu, F., Nishioka, M., Fujita, J., Murota, M., Ohtsuki, Y., Ishida, T., & Kuriyama, S. (2002). Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines. International Journal of Oncology, 20, 31-37. https://doi.org/10.3892/ijo.20.1.31
MLA
Wu, F., Nishioka, M., Fujita, J., Murota, M., Ohtsuki, Y., Ishida, T., Kuriyama, S."Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines". International Journal of Oncology 20.1 (2002): 31-37.
Chicago
Wu, F., Nishioka, M., Fujita, J., Murota, M., Ohtsuki, Y., Ishida, T., Kuriyama, S."Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines". International Journal of Oncology 20, no. 1 (2002): 31-37. https://doi.org/10.3892/ijo.20.1.31