Tributyrin-induced differentiation promotes apoptosis of LS 174T colon cancer cells in vitro

  • Authors:
    • Claudia P. Schroder
    • H. Rainer Maurer
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.195
  • Pages: 195-200
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Tributyrin (glyceryl tributyrate, TB) is known to induce malignant cells to differentiate followed by arrest of cell growth and death via apoptosis. We investigated the effects of TB on the distribution of cell cycle phases, differentiation as measured by alkaline phosphatase activity (ALP), and apoptosis of LS 174T colon cancer cells expressed by morphological changes, externalization of phosphatidylserine and stimulation of various caspases. TB (0.6 mM) reduced the proliferation by a 5-fold decrease of tumor cells in the S-phase and 1.3-fold increase in the G2/M-phase of cell cycle after 24 h of incubation. The ALP activity was enhanced in a dose-dependent manner up to 180-fold by 1 mM TB. Apoptosis was seen only above 0.6 mM TB (5-fold increase). Studies with caspase inhibitors revealed that TB mediated cell death was linked to up-regulation of caspases 3 and 8. Our results indicate that TB-induced differentiation promotes apoptosis in LS 174T cells and may explain the mode of action of TB finally resulting in an arrest of tumor cell growth.

Related Articles

Journal Cover

January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Schroder, C.P., & Schroder, C.P. (2002). Tributyrin-induced differentiation promotes apoptosis of LS 174T colon cancer cells in vitro. International Journal of Oncology, 20, 195-200. https://doi.org/10.3892/ijo.20.1.195
MLA
Schroder, C. P., Maurer, H. R."Tributyrin-induced differentiation promotes apoptosis of LS 174T colon cancer cells in vitro". International Journal of Oncology 20.1 (2002): 195-200.
Chicago
Schroder, C. P., Maurer, H. R."Tributyrin-induced differentiation promotes apoptosis of LS 174T colon cancer cells in vitro". International Journal of Oncology 20, no. 1 (2002): 195-200. https://doi.org/10.3892/ijo.20.1.195