PAR-1- and PAR-3-type thrombin receptor expression in primary cultures of human renal cell carcinoma cells

  • Authors:
    • R. Kaufmann
    • U. Junker
    • K. Nuske
    • M. Westermann
    • P. Henklein
    • J. Scheele
    • K. Junker
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.177
  • Pages: 177-180
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Abstract

In this study, we report coexpression of proteinase-activated receptor (PAR)-1- and PAR-3-type thrombin receptors in primary cultures obtained from surgically resected specimens of renal cell carcinomas (RCCs). Receptor expression on RNA level was evaluated by using the RT-PCR technique. Results demonstrated the presence of mRNA encoding PAR-1 and PAR-3, but mRNA encoding PAR-4 could not be found in human RCC cells. The expression of PAR-1 and PAR-3 on protein level was investigated with confocal laser fluorescence and freeze-fracture electron microscopy. Both thrombin receptor types were localized on the cell membrane but were also found on intracellular compartments of RCC cells. On the outer cell membrane, clustering of PAR-1 and PAR-3 molecules was partly observed. This is the first study demonstrating presence of both PAR-1 and PAR-3 in human carcinoma cells.

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January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kaufmann, R., Junker, U., Nuske, K., Westermann, M., Henklein, P., Scheele, J., & Junker, K. (2002). PAR-1- and PAR-3-type thrombin receptor expression in primary cultures of human renal cell carcinoma cells. International Journal of Oncology, 20, 177-180. https://doi.org/10.3892/ijo.20.1.177
MLA
Kaufmann, R., Junker, U., Nuske, K., Westermann, M., Henklein, P., Scheele, J., Junker, K."PAR-1- and PAR-3-type thrombin receptor expression in primary cultures of human renal cell carcinoma cells". International Journal of Oncology 20.1 (2002): 177-180.
Chicago
Kaufmann, R., Junker, U., Nuske, K., Westermann, M., Henklein, P., Scheele, J., Junker, K."PAR-1- and PAR-3-type thrombin receptor expression in primary cultures of human renal cell carcinoma cells". International Journal of Oncology 20, no. 1 (2002): 177-180. https://doi.org/10.3892/ijo.20.1.177